Departamento de Microbiología y Genética, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Farmacia, Universidad de Salamanca, C/Río Duero 12, Campus de Villamayor, 37185 Salamanca, Spain.
J Plant Physiol. 2011 Jul 15;168(11):1295-302. doi: 10.1016/j.jplph.2011.01.027. Epub 2011 Apr 3.
Using the TrichoEST database, generated in a previous functional genomics project from the beneficial filamentous fungus Trichoderma harzianum, a gene named Thkel1, which codes for a putative kelch-repeat protein, was isolated and characterized. Silencing of this gene in T. harzianum leads to a reduction of glucosidase activity and mycelial growth under abiotic stress conditions. Expression of this gene in Arabidopsis enhances plant tolerance to salt and osmotic stresses, accompanied by an increase in glucosidase activity and a reduction of abscisic acid levels compared to those observed in wild-type plants. Data presented throughout this article suggest the high value of T. harzianum as a source of genes able to facilitate the achievement of producing plants resistant to abiotic stresses without alteration of their phenotype.
利用 Trichoderma harzianum 有益丝状真菌在前一个功能基因组学项目中生成的 TrichoEST 数据库，分离并鉴定了一个名为 Thkel1 的基因，该基因编码一个假定的 Kelch 重复蛋白。在 T. harzianum 中沉默该基因会导致在非生物胁迫条件下葡萄糖苷酶活性和菌丝生长减少。该基因在拟南芥中的表达增强了植物对盐和渗透胁迫的耐受性，与野生型植物相比，葡萄糖苷酶活性增加，脱落酸水平降低。本文提供的所有数据表明，T. harzianum 作为一种能够促进生产抗非生物胁迫植物而不改变其表型的基因来源具有很高的价值。
