Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Gyeonggi-do 463-712, Republic of Korea.
Mol Med Rep. 2011 Mar-Apr;4(2):383-7. doi: 10.3892/mmr.2011.430. Epub 2011 Jan 25.
Abnormal expression of let-7a microRNA (miRNA) in non-small cell lung cancer (NSCLC) cells and tissue has been previously reported. Our objective was to investigate whether let-7a miRNA is aberrantly expressed in the blood of NSCLC patients. Using real-time PCR (RT-PCR), we analyzed let-7a miRNA in archived whole blood from 65 participants, 35 of whom had NSCLC and 30 of whom did not. Using RT-PCR, we also investigated the expression of let-7a miRNA in NSCLC cell lines (A549 and HCC 1588), a normal human lung fibroblast cell line (WI-38) and in 40 human NSCLC tissues. The 2(-ddCt) of let-7a miRNA in the blood of normal subjects and those with NSCLC was 3242.49±355.28 and 747.85±177.74, respectively. The relative expression of let-7a miRNA in the A549 and HCC 1588 cancer cell lines was approximately 0.3 and 0.35, respectively, compared to WI-38 cells. The 2(-ddCt) of let-7a miRNA in the normal human lung tissues and human NSCLC tissues was 42.30±3.98 and 27.73±3.86, respectively. Let-7a miRNAs were under-expressed in the blood of NSCLC patients, as well as NSCLC cells and NSCLC tissues, compared to normal controls. The possibility of using let-7a miRNA as a serologic marker for lung cancer warrants further study.
先前已有报道称，非小细胞肺癌（NSCLC）细胞和组织中 let-7a 微 RNA（miRNA）的表达异常。我们的目的是研究 let-7a miRNA 是否在 NSCLC 患者的血液中异常表达。使用实时 PCR（RT-PCR），我们分析了 65 名参与者的存档全血中的 let-7a miRNA，其中 35 名患有 NSCLC，30 名未患有 NSCLC。我们还使用 RT-PCR 研究了 let-7a miRNA 在 NSCLC 细胞系（A549 和 HCC 1588）、正常人类肺成纤维细胞系（WI-38）和 40 个人类 NSCLC 组织中的表达。正常受试者和 NSCLC 受试者血液中 let-7a miRNA 的 2(-ddCt)分别为 3242.49±355.28 和 747.85±177.74。与 WI-38 细胞相比，A549 和 HCC 1588 癌细胞系中 let-7a miRNA 的相对表达量分别约为 0.3 和 0.35。正常人类肺组织和人类 NSCLC 组织中 let-7a miRNA 的 2(-ddCt)分别为 42.30±3.98 和 27.73±3.86。与正常对照相比，NSCLC 患者的血液、NSCLC 细胞和 NSCLC 组织中 let-7a miRNA 的表达均下调。let-7a miRNA 作为肺癌血清标志物的可能性值得进一步研究。
