Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel.
Inflamm Res. 2011 Aug;60(8):745-50. doi: 10.1007/s00011-011-0328-3. Epub 2011 Apr 5.
The induction of sputum is a safe, noninvasive method of studying airway inflammation in asthma, but the method of analyzing the samples is laborious and requires well-trained technicians using highly specialized laboratory equipment. We introduce a shorter and simpler modification of the process for identifying eosinophilic inflammation from induced sputum (IS) samples.
Samples of 81 patients referred for IS assessment of respiratory diseases were studied. Four different assessment approaches were studied in comparison with the conventional method of selecting plugs proposed by the European Respiratory Society/American Thoracic Society task force.
One modification of the conventional method of IS processing emerged as simpler to execute and less technologically demanding than the original one (13.0 ± 18.2 vs. 15.4 ± 22.4% eosinophils, P = 0.19).
The simpler approach should now encourage the use of IS as a convenient procedure for evaluating eosinophilic inflammation in less sophisticated laboratories.
痰液诱导是一种安全、非侵入性的研究哮喘气道炎症的方法，但样本的分析方法繁琐，需要经过良好培训的技术人员使用高度专业化的实验室设备。我们引入了一种更短、更简单的痰液（IS）样本中嗜酸性粒细胞炎症鉴定方法的改进。
对 81 名因呼吸道疾病而接受 IS 评估的患者进行了样本研究。与欧洲呼吸学会/美国胸科学会工作组提出的传统 IS 处理中选择塞子的方法相比，研究了四种不同的评估方法。
IS 处理的一种改进方法比传统方法更简单，技术要求也更低（13.0±18.2%与 15.4±22.4%嗜酸性粒细胞，P=0.19）。
更简单的方法现在应该鼓励将 IS 作为一种评估欠发达实验室中嗜酸性粒细胞炎症的便捷程序。
