Signorini M, Bergamini C M
Istituto di Chimica Biologica dell'Università, Ferrara, Italy.
Biochem Biophys Res Commun. 1990 Oct 30;172(2):919-24. doi: 10.1016/0006-291x(90)90763-d.
During irradiation in the presence of decavanadate, the subunits of phosphofructokinase underwent progressive degradation to a fragment of about 78,000 daltons. This cleavage pattern was altered when the photoirradiation was performed in the presence of monomeric vanadate with formation of several smaller peptides. The specificity of the decavanadate induced cleavage was proved by the resistance of other enzymes to the treatment and by the effects of phosphofructokinase ligands. During irradiation, the activity of the enzyme declined. Differences between the rate of inactivation and of cleavage of enzyme subunits suggest the occurrence of multiple processes.
在十钒酸盐存在下进行辐照时，磷酸果糖激酶的亚基逐渐降解为约78,000道尔顿的片段。当在单体钒酸盐存在下进行光辐照并形成几种较小的肽时，这种切割模式发生了改变。十钒酸盐诱导切割的特异性通过其他酶对该处理的抗性以及磷酸果糖激酶配体的作用得到证明。在辐照过程中，酶的活性下降。酶亚基失活速率和切割速率之间的差异表明存在多个过程。
