A rapid fluorometric assay of angiotensin-converting enzyme.
作者信息
Hayakari M, Kondo Y
出版信息
Tohoku J Exp Med. 1977 Aug;122(4):313-20. doi: 10.1620/tjem.122.313.
PMID:21470
Abstract
A rapid, simple and sensitive method is described for the fluorometric assay of angiotesin-converting enzyme using Fluorescamine. The critical factors such as optimal pH, incubation time, chloride ion, and inactivation by EDTA and 8-hydroxyquinoline were examined. The Km value for hippuryl-L-histidyl-L-leucine was 0.5 mM. This method was applied to the assay of angiotensin-converting enzyme in the rat serum and the reproducible values were obtained with a 10 mul of the rat serum.
摘要
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