Department of Pathology, School of Medicine, Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, Jiangsu Province, China.
Tissue Cell. 2011 Jun;43(3):201-6. doi: 10.1016/j.tice.2011.03.002. Epub 2011 Apr 5.
To compare the differences in MUC2 and MUC5AC mRNA among four colon cancer cell lines and to identify the best in vitro models for studying mucin expression, quantitative real-time polymerase chain reaction was used to measure the expression of MUC2 and MUC5AC mRNA in Caco-2, HT29, LoVo, and LS174T cell lines. The levels of MUC2 mRNA expression in the four colon cancer cell lines ranked in order of mRNA abundance were: LS174T>LoVo>HT-29>Caco-2. In contrast to MUC2, the abundances of MUC5AC mRNA were in the order: Caco-2>HT-29>LS174T>LoVo. Caco-2 (highest level of MUC5AC mRNA) and LS174T (highest level of MUC2 mRNA) were used to investigate the phenotypes. Morphologically, Caco-2 cells were larger with low electron density mucus-storing vacuoles, many cell surface microvilli, and no obvious intercellular spaces between cells, compared to LS174T cells. The proliferative and invasive capacities of LS174T cells were significantly higher than those of Caco-2 cells. Caco-2 and LS174T cells provide excellent in vitro models for studying mucin expression in colon cancer.
为了比较四种结肠癌细胞系中 MUC2 和 MUC5AC mRNA 的差异，并鉴定研究黏蛋白表达的最佳体外模型，我们使用定量实时聚合酶链反应测量了 Caco-2、HT29、LoVo 和 LS174T 细胞系中 MUC2 和 MUC5AC mRNA 的表达。四种结肠癌细胞系中 MUC2 mRNA 表达水平的排序为：LS174T>LoVo>HT-29>Caco-2。与 MUC2 相反，MUC5AC mRNA 的丰度排序为：Caco-2>HT-29>LS174T>LoVo。我们使用 Caco-2（MUC5AC mRNA 水平最高）和 LS174T（MUC2 mRNA 水平最高）来研究表型。与 LS174T 细胞相比，Caco-2 细胞形态较大，具有低电子密度的黏液储存空泡、许多细胞表面微绒毛，并且细胞之间没有明显的细胞间隙。LS174T 细胞的增殖和侵袭能力明显高于 Caco-2 细胞。Caco-2 和 LS174T 细胞为研究结肠癌中黏蛋白表达提供了极好的体外模型。
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