Department of Integrative Physiology & Bio-System Control, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Biochem Biophys Res Commun. 2012 Aug 3;424(3):524-9. doi: 10.1016/j.bbrc.2012.06.149. Epub 2012 Jul 6.
Peroxisome proliferator-activated receptor gamma (PPARγ) plays an important role in the differentiation of intestinal cells and tissues. Our previous reports indicate that PPARγ is expressed at considerable levels in human colon cancer cells. This suggests that PPARγ expression may be an important factor for cell growth regulation in colon cancer. In this study, we investigated PPARγ expression in 4 human colon cancer cell lines, HT-29, LOVO, DLD-1, and Caco-2. Real-time polymerase chain reaction (PCR) and Western blot analysis revealed that the relative levels of PPARγ mRNA and protein in these cells were in the order HT-29>LOVO>Caco-2>DLD-1. We also found that PPARγ overexpression promoted cell growth inhibition in PPARγ lower-expressing cell lines (Caco-2 and DLD-1), but not in higher-expressing cells (HT-29 and LOVO). We observed a correlation between the level of PPARγ expression and the cells' sensitivity for proliferation.
过氧化物酶体增殖物激活受体γ（PPARγ）在肠道细胞和组织的分化中发挥重要作用。我们之前的报告表明，PPARγ在人结肠癌细胞中表达水平相当高。这表明 PPARγ 的表达可能是结肠癌细胞生长调节的一个重要因素。在这项研究中，我们研究了 4 个人结肠癌细胞系 HT-29、LOVO、DLD-1 和 Caco-2 中的 PPARγ 表达。实时聚合酶链反应（PCR）和 Western blot 分析显示，这些细胞中 PPARγ mRNA 和蛋白的相对水平依次为 HT-29>LOVO>Caco-2>DLD-1。我们还发现，PPARγ 过表达促进了 PPARγ 低表达细胞系（Caco-2 和 DLD-1）的细胞生长抑制，但对高表达细胞系（HT-29 和 LOVO）没有作用。我们观察到 PPARγ 表达水平与细胞增殖敏感性之间存在相关性。
