人菌状乳头细胞的培养特性。
Characterization of human fungiform papillae cells in culture.
机构信息
Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104-3308, USA.
出版信息
Chem Senses. 2011 Sep;36(7):601-12. doi: 10.1093/chemse/bjr012. Epub 2011 Apr 6.
Abstract
The ability to maintain human fungiform papillae cells in culture for multiple cell cycles would be of considerable utility for characterizing the molecular, regenerative, and functional properties of these unique sensory cells. Here we describe a method for enzymatically isolating human cells from fungiform papillae obtained by biopsy and maintaining them in culture for more than 7 passages (7 months) without loss of viability and while retaining many of the functional properties of acutely isolated taste cells. Cells in these cultures exhibited increases in intracellular calcium when stimulated with perceptually appropriate concentrations of several taste stimuli, indicating that at least some of the native signaling pathways were present. This system can provide a useful model for molecular studies of the proliferation, differentiation, and physiological function of human fungiform papillae cells.
摘要
如果能够在培养中维持人类味乳头细胞的多个细胞周期，将对这些独特的感觉细胞的分子、再生和功能特性的研究具有重要的应用价值。本文描述了一种从活检获得的味乳头中酶解分离人类细胞并在培养中维持超过 7 个传代（7 个月）的方法，在此过程中细胞保持活力并保留了急性分离味觉细胞的许多功能特性。当用几种味觉刺激物的感知适当浓度刺激这些培养物中的细胞时，细胞内钙离子增加，表明至少存在一些天然的信号通路。该系统可为研究人类味乳头细胞的增殖、分化和生理功能的分子研究提供一个有用的模型。
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本文引用的文献
1
Stem and progenitor cell compartments within adult mouse taste buds.成年小鼠味蕾中的干细胞和祖细胞隔室。
Eur J Neurosci. 2010 May;31(9):1549-60. doi: 10.1111/j.1460-9568.2010.07184.x.
2
Direct conversion of fibroblasts to functional neurons by defined factors.通过定义因子将成纤维细胞直接转化为功能性神经元。
Nature. 2010 Feb 25;463(7284):1035-41. doi: 10.1038/nature08797. Epub 2010 Jan 27.
3
TGF-beta3 is expressed in taste buds and inhibits proliferation of primary cultured taste epithelial cells.TGF-β3 在味蕾中表达，并抑制原代培养的味觉上皮细胞的增殖。
In Vitro Cell Dev Biol Anim. 2010 Jan;46(1):36-44. doi: 10.1007/s11626-009-9239-9. Epub 2009 Sep 29.
4
Taste signaling elements expressed in gut enteroendocrine cells regulate nutrient-responsive secretion of gut hormones.肠道肠内分泌细胞中表达的味觉信号元件调节肠道激素的营养反应性分泌。
Am J Clin Nutr. 2009 Sep;90(3):822S-825S. doi: 10.3945/ajcn.2009.27462T. Epub 2009 Jul 1.
5
Fate mapping of mammalian embryonic taste bud progenitors.哺乳动物胚胎味蕾祖细胞的命运图谱。
Development. 2009 May;136(9):1519-28. doi: 10.1242/dev.029090.
6
Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors.胶质母细胞瘤细胞的神经分化停滞和间充质高级分化——与神经祖细胞的对比研究
BMC Cancer. 2009 Feb 14;9:54. doi: 10.1186/1471-2407-9-54.
7
The calcium-sensing receptor in taste tissue.味觉组织中的钙敏感受体。
Biochem Biophys Res Commun. 2009 Jan 16;378(3):414-8. doi: 10.1016/j.bbrc.2008.11.060. Epub 2008 Dec 3.
8
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9
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Neuroscience. 2009 Jan 23;158(2):642-53. doi: 10.1016/j.neuroscience.2008.09.059. Epub 2008 Oct 17.
10
Culture of endodermal stem/progenitor cells of the mouse tongue.小鼠舌内胚层干/祖细胞的培养。
In Vitro Cell Dev Biol Anim. 2009 Jan-Feb;45(1-2):44-54. doi: 10.1007/s11626-008-9149-2. Epub 2008 Oct 2.
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