Cheng L H, Robinson P P
Department of Physiology, Birmingham University Medical School, U.K.
Arch Oral Biol. 1991;36(8):583-9. doi: 10.1016/0003-9969(91)90108-7.
Investigations on monkeys have shown that the application of the acidic dye Ponceau S red or the basic dye Alcian blue to the tongue surface facilitates identification of fungiform papillae and taste buds. Both of these dyes were now used in varying degrees of acidity on fixed and unfixed human cadaveric tongues in an attempt to determine the regional distribution of papillae and buds. Satisfactory staining was obtained with acidic Ponceau S red in 10% formalin and 10% trichloracetic acid (pH 2.5). For six tongues, the number of fungiform papillae ranged from 171 to 253 (mean 195) and these were located predominantly at the tip. Of the fungiform papillae, 67% had no staining of taste bud pores. The average number of visible taste pores on the other fungiform papillae was 3 (range 1-21). The correlation between the number of stained taste pores and underlying taste buds was confirmed using serial histological sections of 90 fungiform papillae. This work has shown that a mean of 193 taste buds are carried on fungiform papillae of the human tongue and that 87% of these are located in the anterior 2 cm.
对猴子的研究表明，将酸性染料丽春红S或碱性染料阿尔辛蓝应用于舌表面，有助于识别菌状乳头和味蕾。现在，这两种染料被用于不同酸度的固定和未固定的人类尸体舌头，以试图确定乳头和味蕾的区域分布。在10%福尔马林和10%三氯乙酸（pH 2.5）中使用酸性丽春红S获得了满意的染色效果。对于六个舌头，菌状乳头的数量在171至253个之间（平均195个），且主要位于舌尖。在菌状乳头中，67%的味蕾孔没有染色。其他菌状乳头可见味蕾孔的平均数量为3个（范围为1至21个）。使用90个菌状乳头的连续组织切片证实了染色味蕾孔数量与潜在味蕾之间的相关性。这项工作表明，人类舌头的菌状乳头平均携带193个味蕾，其中87%位于前2厘米处。
