Department of Plant Pathology, Division of Agriculture, University of Arkansas, Fayetteville, AR 72701, USA.
USDA-ARS Horticultural Crops Research Laboratory, Corvallis, OR 97330, USA.
J Gen Virol. 2011 Jul;92(Pt 7):1727-1732. doi: 10.1099/vir.0.031146-0. Epub 2011 Apr 6.
Rose rosette was first described in the early 1940s and it has emerged as one of the most devastating diseases of roses. Although it has been 70 years since the disease description, the rosette agent is yet to be characterized. In this communication, we identify and characterize the putative causal agent of the disease, a negative-sense RNA virus and new member of the genus Emaravirus. The virus was detected in 84/84 rose rosette-affected plants collected from the eastern half of the USA, but not in any of 30 symptomless plants tested. The strong correlation between virus and disease is a good indication that the virus, provisionally named Rose rosette virus, is the causal agent of the disease. Diversity studies using two virus proteins, p3 and p4, demonstrated that the virus has low diversity between isolates as they share nucleotide identities ranging from 97 to 99%.
玫瑰卷叶病于 20 世纪 40 年代初首次被描述，它已成为玫瑰最具破坏性的疾病之一。尽管自疾病描述以来已经过去了 70 年，但卷叶病的致病因子仍未被确定。在本研究中，我们鉴定并描述了该疾病的潜在致病因子，即一种负义 RNA 病毒，也是 Emaravirus 属的一个新成员。该病毒在从美国东半部采集的 84/84 株受玫瑰卷叶病影响的植物中被检测到，但在 30 株无症状植物中未被检测到。病毒与疾病之间的强相关性表明，该病毒，暂命名为玫瑰卷叶病毒，是该疾病的致病因子。使用两种病毒蛋白（p3 和 p4）进行的多样性研究表明，病毒在不同分离株之间的多样性较低，因为它们的核苷酸同一性范围为 97%至 99%。
