Division of Biochemistry, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283 Japan.
In Vivo. 2011 Mar-Apr;25(2):157-63.
Calcium signaling is important in muscular cells and abnormal Ca(2+) handling results in muscle damage. Caldecrin is a serum calcium-decreasing factor purified from pancreas. It is a chymotrypsin-type secretory protease, whereas the serum calcium-decreasing activity does not depend on its protease activity. Here, we evaluated the effect of caldecrin on dystrophia muscularis (dy/dy) mice.
Caldecrin gene in hemagglutinating virus of Japan envelop vector (10 μg/head) was single injected in the femoral muscle or daily intraperitoneal administration of caldecrin (100 μg/kg body weight) was given for 4 days in dy/dy mice. Progression of muscular dystrophy was assessed by blood urea nitrogen and Evans blue dye penetration assay.
Ectopic expression of the caldecrin gene in the muscle of dy/dy mice reduced the level of blood urea nitrogen and improved the dystrophic progression similarly to that of caldecrin-injected mice.
Caldecrin treatment is effective for preventing muscular dystrophy.
钙信号在肌肉细胞中很重要，异常的钙处理会导致肌肉损伤。钙调节蛋白是从胰腺中纯化出来的血清钙降低因子。它是一种胰凝乳蛋白酶型分泌蛋白酶，而血清钙降低活性不依赖于其蛋白酶活性。在这里，我们评估了钙调节蛋白对肌肉营养不良症（dy/dy）小鼠的影响。
将日本血凝病毒包膜载体中的钙调节蛋白基因（10μg/头）单次注射到小鼠的股四头肌中，或在 dy/dy 小鼠中连续 4 天每天腹腔内给予钙调节蛋白（100μg/kg 体重）。通过血尿素氮和 Evans 蓝染料渗透测定评估肌肉营养不良的进展。
钙调节蛋白基因在 dy/dy 小鼠肌肉中的异位表达降低了血尿素氮水平，并改善了肌肉营养不良的进展，与钙调节蛋白注射小鼠相似。
钙调节蛋白治疗可有效预防肌肉营养不良。
