Fischer H, Hedlund G, Kalland T, Sjögren H O, Dohlsten M
Wallenberg Laboratory, Department of Tumor Immunology, University of Lund, Sweden.
J Immunol. 1990 Dec 1;145(11):3767-72.
The lymphokines IL-2 and IL-4 promoted the growth of human PHA-triggered T cells, but only IL-2 induced the production of IFN-gamma and TNF. The addition of purified monocytes strongly enhanced the production of IFN-gamma in IL-2-stimulated T cell cultures but did not influence the production of TNF or the level of T cell proliferation. The addition of IL-1 to T cells activated by PHA and optimal concentrations of IL-2 resulted in a strong induction of IFN-gamma production but had no influence on TNF production or T cell proliferation. IL-6 did not influence IFN-gamma or TNF production or T cell proliferation induced by PHA-IL-2 and did not modulate IL-1-induced IFN-gamma production. The production of IFN-gamma by CD4+ 45R+ Th cells was strongly enhanced by IL-1, whereas CD8+ T cells were less responsive to IL-1 and CD4+ 45R+ T cells were unresponsive to IL-1. We demonstrate, at the clonal level, that the optimal production of IFN-gamma by human Th cells requires both IL-1 and IL-2, whereas the production of TNF and T cell proliferation are induced by IL-2 alone. We suggest that IL-1 acts as a second signal for IFN-gamma production and that it may have an important function in regulating the pattern of lymphokines produced by T cell subsets during activation.
淋巴因子白细胞介素-2（IL-2）和白细胞介素-4（IL-4）可促进人PHA激活的T细胞生长，但只有IL-2能诱导γ干扰素（IFN-γ）和肿瘤坏死因子（TNF）的产生。加入纯化的单核细胞可显著增强IL-2刺激的T细胞培养物中IFN-γ的产生，但不影响TNF的产生或T细胞增殖水平。向由PHA和最佳浓度IL-2激活的T细胞中加入IL-1可强烈诱导IFN-γ的产生，但对TNF的产生或T细胞增殖没有影响。IL-6不影响PHA-IL-2诱导的IFN-γ或TNF的产生或T细胞增殖，也不调节IL-1诱导的IFN-γ产生。IL-1可强烈增强CD4+ 45R+ Th细胞产生IFN-γ，而CD8+ T细胞对IL-1反应较弱，CD4+ 45R+ T细胞对IL-1无反应。我们在克隆水平上证明，人Th细胞最佳产生IFN-γ需要IL-1和IL-2两者，而TNF的产生和T细胞增殖仅由IL-2诱导。我们认为IL-1作为IFN-γ产生的第二信号，并且它可能在调节激活过程中T细胞亚群产生的淋巴因子模式方面具有重要功能。
