VTT Technical Research Centre of Finland, P.O. Box 1300, 33101 Tampere, Finland.
Anal Bioanal Chem. 2011 May;400(5):1387-96. doi: 10.1007/s00216-011-4871-0. Epub 2011 Apr 7.
Nanomolar quantities of single-stranded DNA products ~100 nucleotides long can be detected in diluted 1% serum by surface plasmon resonance (SPR) and film bulk acoustic resonators (FBARs). We have used a novel FBAR sensor in parallel with SPR and obtained promising results with both the acoustic and the optical device. Oligonucleotides and a repellent lipoamide, Lipa-DEA, were allowed to assemble on the sensor chip surfaces for only 15 min by dispensing. Lipa-DEA surrounds the analyte-binding probes on the surface and effectively reduces the non-specific binding of bovine serum albumin and non-complementary strands. In a highly diluted serum matrix, the non-specific binding is, however, a hindrance, and the background response must be reduced. Nanomolar concentrations of short complementary oligos could be detected in buffer, whereas the response was too low to be measured in serum. DNA strands that are approximately 100 base pairs long at concentrations as low as 1-nM could be detected both in buffer and in 1% serum by both SPR and the FBAR resonator.
通过表面等离子体共振（SPR）和薄膜体声波谐振器（FBAR），可以在稀释至 1%的血清中检测到~100 个核苷酸长的单链 DNA 产物的纳摩尔数量。我们使用一种新型的 FBAR 传感器与 SPR 平行使用，并通过声学和光学设备都获得了有前景的结果。寡核苷酸和一种驱避性脂酰胺 Lipa-DEA 通过分配仅在传感器芯片表面上组装 15 分钟。Lipa-DEA 围绕表面上的分析物结合探针，并有效地减少牛血清白蛋白和非互补链的非特异性结合。然而，在高度稀释的血清基质中，非特异性结合是一个障碍，必须减少背景响应。在缓冲液中可以检测到纳摩尔浓度的短互补寡核苷酸，而在血清中则响应太低而无法测量。通过 SPR 和 FBAR 谐振器，在缓冲液和 1%的血清中，浓度低至 1 nM 的大约 100 个碱基对长的 DNA 链都可以被检测到。
