Santos-González Mónica, López-Miranda José, Pérez-Jiménez Francisco, Navas Plácido, Villalba José M
Departamento de Biología Celular, Fisiología e Inmunología, University of Córdoba, Spain.
Age (Dordr). 2012 Apr;34(2):341-58. doi: 10.1007/s11357-011-9239-z. Epub 2011 Apr 7.
Fatty acids and other components of the diet may modulate, among others, mechanisms involved in homeostasis, aging, and age-related diseases. Using a proteomic approach, we have studied how dietary oil affected plasma proteins in young (6 months) or old (24 months) rats fed lifelong with two experimental diets enriched in either sunflower or virgin olive oil. After the depletion of the most abundant proteins, levels of less abundant proteins were studied using two-dimensional electrophoresis and mass spectrometry. Our results showed that compared with the sunflower oil diet, the virgin olive oil diet induced significant decreases of plasma levels of acute phase proteins such as inter-alpha inhibitor H4P heavy chain (at 6 months), hemopexin precursor (at 6 and 24 months), preprohaptoglobin precursor (at 6 and 24 months), and α-2-HS glycoprotein (at 6 and 24 months); antioxidant proteins such as type II peroxiredoxin (at 24 months); proteins related with coagulation such as fibrinogen γ-chain precursor (at 24 months), T-kininogen 1 precursor (at 6 and 24 months), and apolipoprotein H (at 6 and 24 months); or with lipid metabolism and transport such as apolipoprotein E (at 6 and 24 months) and apolipoprotein A-IV (at 24 months). The same diet increased the levels of apolipoprotein A-1 (at 6 and 24 months), diminishing in general the changes that occurred with age. Our unbiased analysis reinforces the beneficial role of a diet rich in virgin olive oil compared with a diet rich in sunflower oil, modulating inflammation, homeostasis, oxidative stress, and cardiovascular risk during aging.
饮食中的脂肪酸和其他成分可能会调节体内平衡、衰老以及与年龄相关疾病所涉及的多种机制。我们采用蛋白质组学方法，研究了终生喂食富含葵花籽油或初榨橄榄油的两种实验性饮食的年轻（6个月）或年老（24个月）大鼠中，膳食油如何影响血浆蛋白。在去除最丰富的蛋白质后，使用二维电泳和质谱法研究了较不丰富蛋白质的水平。我们的结果表明，与葵花籽油饮食相比，初榨橄榄油饮食可显著降低急性期蛋白的血浆水平，如α-抑制剂H4P重链（6个月时）、血红素结合蛋白前体（6个月和24个月时）、前原触珠蛋白前体（6个月和24个月时）以及α-2-HS糖蛋白（6个月和24个月时）；抗氧化蛋白，如II型过氧化物酶（24个月时）；与凝血相关的蛋白，如纤维蛋白原γ链前体（24个月时）、T-激肽原1前体（6个月和24个月时）以及载脂蛋白H（6个月和24个月时）；或与脂质代谢和转运相关的蛋白，如载脂蛋白E（6个月和24个月时）和载脂蛋白A-IV（24个月时）。相同的饮食增加了载脂蛋白A-1的水平（6个月和24个月时），总体上减少了随年龄发生的变化。我们的无偏分析强化了富含初榨橄榄油的饮食与富含葵花籽油的饮食相比的有益作用，在衰老过程中调节炎症、体内平衡、氧化应激和心血管风险。
