Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
J Am Soc Mass Spectrom. 2011 Jan;22(1):67-74. doi: 10.1007/s13361-010-0015-6. Epub 2011 Jan 28.
Herein we describe a new method, targeted enhanced multiply charged scans (tEMC), for the quantification of therapeutic peptides in tandem mass spectrometry on the linear ion trap mass spectrometer. Therapeutic peptides with chain lengths between eight and 39 amino acid residues and charge states from 2+ to 6+ were used to evaluate and illustrate the method which relies on the ability to separate ions trapped in a linear ion trap according to their charges. In particular, interference from singly charged ions on multiply charged ions can be effectively minimized. The method requires optimization of relatively few parameters, the most important of which being the exit lens barrier (EXB) voltage, thereby offering substantial time saving in a high-throughput quantification environment that currently relies on selected reaction monitoring.
在此，我们描述了一种新方法，靶向增强多电荷扫描（tEMC），用于在线性离子阱质谱仪上进行串联质谱法对治疗性肽的定量分析。我们使用了长度在 8 到 39 个氨基酸残基之间且电荷状态为 2+ 到 6+的治疗性肽来评估和说明该方法，该方法依赖于根据其电荷分离在线性离子阱中捕获的离子的能力。特别是，可以有效地最小化单电荷离子对多电荷离子的干扰。该方法需要优化相对较少的参数，其中最重要的是出口透镜屏障（EXB）电压，因此在目前依赖于选择反应监测的高通量定量环境中可以节省大量时间。
