Endocrine Oncology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Cancer. 2011 Apr 15;117(8):1630-9. doi: 10.1002/cncr.25724. Epub 2010 Nov 8.
The authors are interested in identifying molecular markers that can aid in the diagnosis of adrenocortical carcinoma (ACC). The aim of this study was to identify microRNAs (miRNAs or miRs) that are differentially expressed in malignant adrenocortical tumors as compared with benign tumors and assess their potential as diagnostic predictors.
Differentially expressed miRNAs were identified using microarray profiling of adrenocortical tumors and validated by quantitative real-time RT-PCR.
Microarray profiling in benign and primary malignant adrenocortical tumors revealed several significant differences between these histological groups. By using directed quantitative RT-PCR analysis on a subset of these differentially expressed miRNAs, the authors determined that miRs -100, -125b, and -195 were significantly down-regulated, whereas miR-483-5p was significantly up-regulated in malignant as compared with benign tumors. Furthermore, the current study shows that miR-483-5p expression can accurately categorize tumors as benign or malignant.
The authors identified 4 miRNAs that are dysregulated in adrenocortical carcinoma. The high expression of one of these, miR-483-5p, appears to be a defining characteristic of adrenocortical malignancies, and can thus be used to accurately distinguish between benign and malignant adrenocortical tumors.
作者们对鉴定可辅助诊断肾上腺皮质癌（ACC）的分子标志物很感兴趣。本研究旨在鉴定与良性肿瘤相比在恶性肾上腺皮质肿瘤中差异表达的 microRNAs（miRNAs 或 miRs），并评估其作为诊断预测因子的潜力。
使用肾上腺皮质肿瘤的微阵列分析鉴定差异表达的 miRNAs，并通过定量实时 RT-PCR 进行验证。
良性和原发性恶性肾上腺皮质肿瘤的微阵列分析显示这些组织学组之间存在多个显著差异。通过对这些差异表达的 miRNAs 中的一部分进行定向定量 RT-PCR 分析，作者确定与良性肿瘤相比，miR-100、miR-125b 和 miR-195 明显下调，而 miR-483-5p 明显上调。此外，本研究表明 miR-483-5p 的表达可以准确地将肿瘤分类为良性或恶性。
作者鉴定出在肾上腺皮质癌中失调的 4 个 miRNAs。其中一种的高表达，miR-483-5p，似乎是肾上腺皮质恶性肿瘤的一个特征，可以用于准确区分良性和恶性肾上腺皮质肿瘤。
