Department of Surgery, University of Vermont/Fletcher Allen Health Care, Burlington, Vermont, USA.
J Surg Oncol. 2011 Sep 1;104(3):260-4. doi: 10.1002/jso.21936. Epub 2011 Apr 6.
Melanoma continues to be a therapeutic challenge for the medical community owing to the scarcity of effective agents available to treat the disease. Honokiol, a traditional Chinese herb, has been proven to have anti-cancer effects in various cell types, therefore we hypothesized it may have similar cytotoxic capabilities against melanoma cells in vitro.
Two cell lines, SK-MEL2 and MeWo, were grown in culture and exposed to increasing doses of Honokiol. Cell proliferation, cytochrome c release into the cytosol, intra-cellular caspase activity, and mitochondrial depolarization were then evaluated after treatment with honokiol.
Melanoma cells in culture underwent cell death, had increased cytosolic cytochrome c, showed greater caspase activity, and demonstrated increased mitochondrial depolarization after treatment when compared to controls.
It appears that honokiol is an effective inhibitor of cultured human melanoma cells.
由于治疗疾病的有效药物稀缺，黑色素瘤仍然是医学界的一个治疗挑战。厚朴酚是一种传统的中草药，已被证明在各种细胞类型中具有抗癌作用，因此我们假设它可能对体外黑色素瘤细胞具有类似的细胞毒性作用。
将两种细胞系 SK-MEL2 和 MeWo 在培养中生长，并暴露于递增剂量的厚朴酚中。然后在用厚朴酚处理后评估细胞增殖、细胞色素 c 向细胞质中的释放、细胞内半胱天冬酶活性和线粒体去极化。
与对照组相比，培养中的黑色素瘤细胞发生细胞死亡，细胞质中的细胞色素 c 增加，半胱天冬酶活性更高，线粒体去极化增加。
厚朴酚似乎是一种有效的体外培养人黑色素瘤细胞抑制剂。
