Department of Environmental Protection Engineering, University of Warmia and Mazury in Olsztyn, Poland.
Environ Technol. 2011 Jan;32(1-2):37-42. doi: 10.1080/09593331003616805.
The possibility of using whey permeate as a raw material for the production of ethanol by continuous fermentation by using Kluyveromyces marxianus was examined. The ethanol formation was investigated as a function of the hydraulic retention time in a UASB reactor. The initial lactose concentration in permeate was 50.0 g L(-1). The hydraulic retention time supplied were 12, 24, 48 h and the daily ethanol formations were 4.46, 8.61, 7.73 g L(-1), respectively. The yield coefficient of ethanol was 0.089 g ethanol g(-1) lactose when the hydraulic retention time was 12 h and raised to 0.325 g g(-1) when the hydraulic retention time was as long as 48 h. The results indicated that in ethanol fermentation the hydraulic retention time should be 24 h to obtain high rates of ethanol formation and to avoid product inhibition.
考察了利用乳清渗透物作为原料,通过连续发酵生产乙醇的可能性,使用马克斯克鲁维酵母(Kluyveromyces marxianus)。考察了在 UASB 反应器中乙醇形成的水力停留时间函数。乳清渗透物中的初始乳糖浓度为 50.0 g/L。提供的水力停留时间分别为 12、24 和 48 h,每天的乙醇产量分别为 4.46、8.61 和 7.73 g/L。当水力停留时间为 12 h 时,乙醇的产率系数为 0.089 g 乙醇/g 乳糖,当水力停留时间长达 48 h 时,提高到 0.325 g/g。结果表明,在乙醇发酵中,水力停留时间应为 24 h,以获得较高的乙醇形成速率,并避免产物抑制。