江苏省中国女性采用第二代杂交捕获法进行人乳头瘤病毒筛查。

Human papilloma virus screening by hybrid capture II in Chinese women of Jiangsu Province.

作者信息

Wang Q W, Xu P Z, Zhang B, Dong Y S, Yang Y Q, Cao F, Yu B

机构信息

Changzhou Woman and Children Health Hospital, Changzhou 213003, Jiangsu Province, China.

出版信息

West Indian Med J. 2010 Oct;59(5):469-72.

PMID:21473391

Abstract

OBJECTIVE

To survey Human Papilloma Virus (HPV) infection in Chinese Women of Jiangsu Province and discuss the relationship between HPV and the biology of cervical cancer.

METHODS

Two thousand, one hundred and fifty-three sexually active women (including 66 cases of cervical cancer) were selected for high-risk human papilloma virus DNA test with Hybrid Capture II (HCII).

RESULTS

The overall HPV prevalence was 32.6% (701/2153) with higher positive rates in cervical carcinoma and Cervical Interstitial Neoplasia (CIN) [93.9% and 54.6%] respectively. For women aged 40-59 years, the overall high-risk HPV prevalence was higher than those of other age groups. Compared with CIN I, the positivity rate and viral load of HPV DNA in CIN III is much higher (80.2% vs. 29.9%, 11.89 vs. 0.53). Ninety-four per cent (64/66) of patients with Cervical cancer were detected to be HPV positive. There was no significant difference in HPV DNA among each clinical stage and pathologic grade. But the positive rates and the value of HPV DNA were higher in the patients with cervical interstitial incursion. Eighty per cent of patients (20/25) could become negative within six months after operation.

CONCLUSIONS

High-risk HPV DNA test is effective in screening for cervical diseases. HCII is an effective method to detect HPV DNA.

摘要

目的

调查江苏省中国女性人乳头瘤病毒（HPV）感染情况，并探讨HPV与宫颈癌生物学特性之间的关系。

方法

选取2153名性活跃女性（包括66例宫颈癌患者），采用第二代杂交捕获法（HCII）进行高危型人乳头瘤病毒DNA检测。

结果

HPV总体感染率为32.6%（701/2153），在宫颈癌和宫颈上皮内瘤变（CIN）中阳性率较高，分别为93.9%和54.6%。40 - 59岁女性高危型HPV总体感染率高于其他年龄组。与CIN I相比，CIN III中HPV DNA的阳性率和病毒载量更高（80.2%对29.9%，11.89对0.53）。94%（64/66）的宫颈癌患者检测出HPV阳性。各临床分期和病理分级之间HPV DNA无显著差异。但宫颈间质浸润患者的HPV DNA阳性率和病毒载量较高。80%的患者（20/25）术后6个月内可转阴。