Seccion de estudios de posgrado e investigación, Escuela Superior de Medicina del IPN, México, DF.
Clin Exp Hypertens. 2011;33(3):167-73. doi: 10.3109/10641963.2010.531843. Epub 2011 Apr 8.
Both gestation and diabetes mellitus (DM) are conditions associated with an increased participation of renin-angiotensin system (RAS) as well as with changes in the vascular response to angiotensin II (Ang II). We sought to establish the impact of gestational diabetes mellitus (GDM) on Ang II- induced vasoconstriction and its correlation with the expression of angiotensin II receptors (AT(1)R, AT(2) R). Female Wistar rats, virgin, or on day 3 after mating, received randomly streptozotocin (STZ) 60 mg/kgor saline ip.Streptozotocin-treated animals developed hyperglycemia (25.6 ± 1.42 mM). Ang II-induced vasoconstriction was evaluated on isolated aortas with (e+) and without (e-) endothelium and the protein expression of AT(1)R and AT(2)R was measured by western blot. Gestational diabetes mellitus significantly increased vasoconstriction with respect to all the other groups. Changes were observed only on e+ vessels. Interestingly, GDM moved AT(1)R: AT(2)R balance towards AT(1) R, while both pregnancy and diabetes towards AT(2)R expression. In conclusion, GDM increases the possibility of an hypertensive complication by an increased AT(1)R expression.
妊娠和糖尿病（DM）均与肾素-血管紧张素系统（RAS）的参与增加以及血管对血管紧张素 II（Ang II）的反应变化有关。我们试图确定妊娠糖尿病（GDM）对 Ang II 诱导的血管收缩的影响及其与血管紧张素 II 受体（AT（1）R、AT（2）R）表达的相关性。雌性 Wistar 大鼠，处女或交配后第 3 天，随机接受链脲佐菌素（STZ）60mg/kg 或腹腔注射生理盐水。STZ 处理的动物出现高血糖（25.6±1.42mM）。通过分离的带有（e+）和不带（e-）内皮的主动脉评估 Ang II 诱导的血管收缩，并通过 Western blot 测量 AT（1）R 和 AT（2）R 的蛋白表达。与所有其他组相比，妊娠期糖尿病显着增加了血管收缩。仅在 e+血管中观察到变化。有趣的是，GDM 将 AT（1）R：AT（2）R 平衡向 AT（1）R 移动，而妊娠和糖尿病均向 AT（2）R 表达移动。总之，GDM 通过增加 AT（1）R 的表达增加了发生高血压并发症的可能性。
