Research Group of Pain and Neuroscience, WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea.
J Ethnopharmacol. 2011 Jun 1;135(3):636-45. doi: 10.1016/j.jep.2011.03.066. Epub 2011 Apr 5.
The leaf of Eriobotrya japonica (Ej) has been used for a long time as an oriental medicine to treat pulmonary inflammatory diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the anti-inflammatory changes of the genes by the leaves of Ej when challenged with LPS using a microarray chip.
A whole transcript genechip (Affymetrix genechip human gene 1.0 ST array) was used. The expression patterns of the significant genes were confirmed by real-time RT-PCR analysis.
The gene array analysis showed that 60 of the 325 genes up-regulated by the LPS when compared to the control were down-regulated by the Ej treatment. Of these 60 genes, the inflammation- and immune response-related genes were especially noted, which indicates that Ej inhibits the induction of the inflammation through LPS stimulation. In addition, 78 of the 158 genes down-regulated by the LPS when compared to the control were up-regulated by the Ej treatment. The regulatory patterns of the representative genes in the real-time RT-PCR correlated with those of the genes shown in the microarray. The Ej extract also inhibited the production of IL-6, TNF-α, and nitrite in the LPS-stimulated cells.
This study showed that the extract of Ej leaves could be used to inhibit the activation of a wide variety of the inflammation-related genes and the inflammatory mediators. It is suggested that the extract of Ej leaves may be applied to alleviate the inflammation of periodontal diseases.
枇杷叶（Ej）长期以来一直被用作东方药物来治疗肺部炎症性疾病。本研究通过微阵列芯片调查了脂多糖（LPS）刺激培养的人牙龈成纤维细胞中的基因表达变化，以及 LPS 刺激时 Ej 叶对基因的抗炎变化。
使用全转录基因芯片（Affymetrix 基因芯片人类基因 1.0 ST 阵列）。通过实时 RT-PCR 分析确认显著基因的表达模式。
基因芯片分析表明，与对照相比，60 个 LPS 上调的 325 个基因被 Ej 处理下调。在这 60 个基因中，特别注意到与炎症和免疫反应相关的基因，这表明 Ej 通过 LPS 刺激抑制炎症的诱导。此外，与对照相比，158 个 LPS 下调的基因中有 78 个被 Ej 处理上调。实时 RT-PCR 中代表性基因的调控模式与微阵列中显示的基因的调控模式相关。Ej 提取物还抑制了 LPS 刺激细胞中 IL-6、TNF-α 和亚硝酸盐的产生。
本研究表明，Ej 叶提取物可用于抑制多种与炎症相关的基因和炎症介质的激活。提示 Ej 叶提取物可用于减轻牙周病的炎症。
