UR1268 BIA, INRA, Rue de la Géraudière, BP 71627, 44316 Nantes, France.
J Proteomics. 2011 Aug 12;74(8):1279-89. doi: 10.1016/j.jprot.2011.03.014. Epub 2011 Apr 5.
Wheat is an important part of the daily diet of millions of people. However, this staple food is also responsible for food allergies. Ancient cultivars of wheat are gaining interest today but nothing is known about their allergenicity. Many wheat proteins have been reported as causative food allergens, including some prolamin-type gluten proteins, and salt soluble proteins of the albumin/globulin (A/G) type. The objective of this work is to obtain information about the allergenicity of the salt soluble A/G fraction of an ancient diploid cultivar compared with a standard hexaploid bread wheat cultivar using 20 sera from patients with wheat allergy. Differences in the IgE reactivity of sera towards the two genotypes were quantified by ELISA. Qualitative differences in IgE-binding proteins were searched after 1D or 2D electrophoresis. For most of the sera, the concentration in A/G specific IgE was higher for the hexaploid T. aestivum (cv Récital) than for the diploid T. monococcum (cv Engrain). The analysis of 2D spots revealed by immunoblotting leads to the identification by mass spectrometry of 39 IgE-binding proteins, some of them unknown until now as wheat allergens. Numerous allergens were identified, differences observed between Engrain and Récital will be discussed.
小麦是数百万人日常饮食的重要组成部分。然而，这种主食也是食物过敏的罪魁祸首。如今，人们对古代小麦品种越来越感兴趣，但对它们的致敏性却一无所知。许多小麦蛋白已被报道为引起食物过敏的过敏原，包括一些醇溶蛋白型谷蛋白和白蛋白/球蛋白（A/G）型盐溶性蛋白。本工作的目的是使用 20 份来自小麦过敏患者的血清，获得与标准六倍体面包小麦品种相比，古代二倍体品种盐溶性 A/G 组分的致敏性信息。通过 ELISA 定量检测血清对两种基因型的 IgE 反应性差异。在 1D 或 2D 电泳后，寻找 IgE 结合蛋白的定性差异。对于大多数血清，六倍体 T. aestivum（cv Récital）的 A/G 特异性 IgE 浓度高于二倍体 T. monococcum（cv Engrain）。免疫印迹分析揭示的 2D 斑点通过质谱鉴定出 39 种 IgE 结合蛋白，其中一些直到现在才被认为是小麦过敏原。鉴定出了许多过敏原，将讨论 Engrain 和 Récital 之间观察到的差异。
