人外泌汗腺汗液中的肾素样（血管紧张素原酶）活性。

Renin-like (angiotensinogenase) activity in human eccrine sweat.

作者信息

Dahlheim H, Emrich H M

出版信息

Pflugers Arch. 1978 Oct 18;377(1):65-7. doi: 10.1007/BF00584375.

Abstract

The presence of renin or renin-like activity (RLA) was demonstrated in human eccrine sweat incubated with purified sheep angiotensinogen, using rat bioassay and angiotensin I radioimmunoassay. Following cholinergic stimulation, sweat RLA was found to range between 0 (unmeasurable) and 266 ng/ml.h, i.e. RLA-values of sweat can be about 10 times higher than those of plasma. Therefore, renin synthesis in sweat glands could be assumed. RLA following activation of beta-adrenergic receptors by the administration of isoprenaline (Aludrin) did not exceed the mean values obtained by cholinergic activation. After beta-adrenergic receptor blockade by propranolol (Dociton), RLA became unmeasurably low. Higher RLA-values were found after local injection of dibutyryl-c-AMP (90--210 ng/ml.h). The results indicate a beta-adrenergic regulation of RLA-release in human sweat glands. Human sweat glands appear to be useful for studying extrarenal renin release.

摘要

采用大鼠生物测定法和血管紧张素I放射免疫测定法，在与纯化的绵羊血管紧张素原一起孵育的人外泌汗腺汗液中证实了肾素或肾素样活性（RLA）的存在。胆碱能刺激后，发现汗液RLA在0（不可测）至266 ng/ml·h之间，即汗液的RLA值可比血浆RLA值高约10倍。因此，可以推测汗腺中存在肾素合成。通过给予异丙肾上腺素（爱喘乐）激活β-肾上腺素能受体后的RLA未超过胆碱能激活所获得的平均值。在普萘洛尔（心得静）阻断β-肾上腺素能受体后，RLA变得低至不可测。局部注射二丁酰环磷腺苷（90-210 ng/ml·h）后发现RLA值更高。结果表明人汗腺中RLA释放存在β-肾上腺素能调节。人汗腺似乎有助于研究肾外肾素释放。