Sato Yukihiro, Sato Hiroyuki, Naka Keisuke, Furuya Satoshi, Tsukiji Haruhisa, Kitagawa Koji, Sonoda Yoshihide, Usui Takanobu, Sakamoto Hirotsugu, Yoshino Sumi, Shimizu Yuko, Takahashi Masaharu, Nagashima Shigeo, Nishizawa Tsutomu, Okamoto Hiroaki
Kamiichi General Hospital, Kamiichi, Toyama, 930-0391, Japan.
Arch Virol. 2011 Aug;156(8):1345-58. doi: 10.1007/s00705-011-0988-x. Epub 2011 Apr 8.
To investigate the nationwide prevalence of hepatitis E virus (HEV) infection and to characterize HEV genomes among Japanese wild boars (Sus scrofa leucomystax), 578 boars captured in 25 prefectures from 2003 to 2010 were studied. Anti-HEV IgG was detected in 8.1%, and HEV RNA in 3.3% of boars. Among the 19 boar HEV isolates obtained from infected boars, 14 isolates (74%) were classified as genotype 3, 4 isolates (21%) as genotype 4, and the remaining isolate (wbJOY_06) was distantly related to all known HEV isolates of genotypes 1-4, differing by 18.4-25.0% and 18.0-24.3% within the 412-nucleotide sequence of ORF1 and ORF2, respectively. A genotype 4 boar HEV isolate (wbJGF_08-1) obtained herein shared 98.6% identity over the entire genome with a human HEV isolate obtained from a patient who developed acute hepatitis after consuming undercooked wild boar meat, suggesting that wild boars are also reservoirs for genotype 4 HEV in humans.
为调查日本野猪（野猪指名亚种）中戊型肝炎病毒（HEV）感染的全国流行情况并对HEV基因组进行特征分析，我们研究了2003年至2010年在25个县捕获的578头野猪。在8.1%的野猪中检测到抗HEV IgG，在3.3%的野猪中检测到HEV RNA。从感染野猪中获得的19株野猪HEV分离株中，14株（74%）被归类为3型，4株（21%）为4型，其余分离株（wbJOY_06）与所有已知的1-4型HEV分离株亲缘关系较远，在ORF1和ORF2的412个核苷酸序列中分别相差18.4-25.0%和18.0-24.3%。在此获得的一株4型野猪HEV分离株（wbJGF_08-1）与从食用未煮熟的野猪肉后发生急性肝炎的患者中分离出的一株人HEV分离株在全基因组上具有98.6%的同一性，这表明野猪也是人类4型HEV的储存宿主。
