Department of Mechanical Engineering, Dankook University, Yong-in, Republic of Korea.
Biotechnol Lett. 2011 Aug;33(8):1675-81. doi: 10.1007/s10529-011-0604-x. Epub 2011 Apr 8.
The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.
使用带有超微电极 (UME) 的纳米探针在亚微米系统中结合微流控系统研究了单细胞的氧气释放。将单个细胞固定在微流控系统中，并将纳米探针插入单个细胞的胞质空间。然后，在固定电位下使用 UME 进行体内电流测定实验和电化学阻抗谱，以在不同光强下检测单个细胞的氧气释放。
