College of Life Science, Sichuan University, 29 Wangjiang Road, 610064 Chengdu, Sichuan, P. R. China.
Z Naturforsch C J Biosci. 2011 Jan-Feb;66(1-2):63-72.
A full-length cDNA encoding a putative diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) was obtained from sunflower (Helianthus annuus L.) seeds. The 1524-bp open reading frame of this cDNA, designated as HaDGAT1, encodes a protein of 507 amino acids with a molecular mass of 58.5 kDa showing high homology to DGAT1 enzymes of other plants. The protein characters, such as a predicted structure with a long N-terminal hydrophilic domain followed by 9 transmembrane domains, acyl-CoA-binding signature, diacylglycerol (DAG)-binding and putative endoplasmic reticulum retrieval motifs (ER-DIR), also indicated that HaDGAT belongs to the DGAT1 family. HaDGAT1 is expressed in all plant tissues especially in developing seeds. Expression of recombinant HaDGAT1 in yeast showed an 1.76-fold increase of total fatty acids, especially unsaturated fatty acids such as palmitoleic acid (enhanced by 86.6%) and oleic acid (enhanced by 81.6%).
从向日葵（Helianthus annuus L.）种子中获得了一个全长 cDNA，编码一个假定的二酰基甘油酰基转移酶（DGAT；EC 2.3.1.20）。该 cDNA 的 1524 个碱基对的开放阅读框，命名为 HaDGAT1，编码一个 507 个氨基酸的蛋白质，与其他植物的 DGAT1 酶具有高度同源性。该蛋白质的特征，如具有长的 N 端亲水结构域，其后是 9 个跨膜结构域、酰基辅酶 A 结合特征、二酰基甘油（DAG）结合和假定的内质网回收基序（ER-DIR），也表明 HaDGAT 属于 DGAT1 家族。HaDGAT1 在所有植物组织中表达，特别是在发育中的种子中。在酵母中表达重组 HaDGAT1 可使总脂肪酸增加 1.76 倍，特别是不饱和脂肪酸，如棕榈油酸（增加 86.6%）和油酸（增加 81.6%）。
