Vollmar A M, Friedrich A, Schulz R
Institute of Pharmacology, Toxicology and Pharmacy, University of Munich, Federal Republic of Germany.
J Androl. 1990 Sep-Oct;11(5):471-5.
Atrial natriuretic peptide (ANP) has recently been suggested to play a role in the regulation of male reproduction. Specifically, it has been shown to affect steroidogenesis in Leydig cells. The data presented here demonstrate that the rat testis also contains immunoreactive, ANP-like material (IR-ANP). Characterization of this material was conducted by high-pressure liquid chromatography (HPLC) analysis employing two different antisera to recognize either the C- or the N-terminals of the ANP prohormone (ANP 1-126). The IR-ANP corresponded to the 15 kd ANP precursor molecule (ANP 1-126), indicating the synthesis of ANP within the testis. This was supported by the detection of ANP messenger RNA transcripts (Northern blot hybridization) in the testis. These data indicate that ANP is expressed in the rat testis and support the notion that it may function in a paracrine and/or autocrine fashion in this organ.
最近有人提出心房利钠肽（ANP）在雄性生殖调节中发挥作用。具体而言，已证明它会影响睾丸间质细胞的类固醇生成。此处呈现的数据表明，大鼠睾丸中也含有免疫反应性的、类似ANP的物质（IR-ANP）。通过高压液相色谱（HPLC）分析对该物质进行了表征，使用两种不同的抗血清来识别ANP前体激素（ANP 1-126）的C端或N端。IR-ANP对应于15 kd的ANP前体分子（ANP 1-126），表明睾丸内合成了ANP。睾丸中检测到ANP信使RNA转录本（Northern印迹杂交）支持了这一点。这些数据表明ANP在大鼠睾丸中表达，并支持其可能在该器官中以旁分泌和/或自分泌方式发挥作用的观点。
