Biosystems and Agricultural Engineering, Michigan State University, East Lansing, Michigan 48824, USA.
J Food Prot. 2011 Apr;74(4):603-9. doi: 10.4315/0362-028X.JFP-10-416.
Pediococcus sp. NRRL B-2354 was investigated as a potential nonpathogenic surrogate for Salmonella enterica serovar Enteritidis phage type 30 (SE PT30) on the surface of almonds subjected to moist-air heating. Both microorganisms were subjected to various time, temperature, and humidity regimens on almonds processed in a computer-controlled, laboratory-scale, moist-air convection oven. Overall, the mean log reductions for Pediococcus sp. were 0.6 log and 1.4 log lower than those for SE PT30 (P < 0.05) at predicted reductions of 3 and 5 log, respectively. Also, the D(ref)-values for Pediococcus sp., calculated using a modified inactivation model (accounting for moisture) for SE PT30 on the surface of almonds subjected to moist-air heating (30 to 90% moisture by volume) were ~30% larger than those for SE PT30. Based on these findings, Pediococcus sp. NRRL B-2354 can be used as a conservative surrogate for SE PT30 during moist-air heating, and this organism is also likely to be an acceptable surrogate for steam heating.
在对经过湿空气加热的杏仁表面进行研究时，发现肠球菌（Pediococcus sp.）NRRL B-2354 可能是沙门氏菌肠炎亚种 30 型（SE PT30）的无致病性替代物。这两种微生物都经过了不同时间、温度和湿度的处理，处理过程是在一个计算机控制的、实验室规模的、湿空气对流烤箱中进行的。总的来说，与 SE PT30 相比（P < 0.05），肠球菌的平均对数减少量分别低 0.6 对数和 1.4 对数，在预测的减少 3 对数和 5 对数时分别为 0.6 对数和 1.4 对数。此外，使用针对在湿空气加热下（体积湿度为 30%至 90%）表面的杏仁的 SE PT30 的改良失活动力学模型（考虑水分）计算出的肠球菌的 D(ref)值比 SE PT30 大约 30%。基于这些发现，肠球菌（Pediococcus sp.）NRRL B-2354 可以在湿空气加热过程中作为 SE PT30 的保守替代物，而且该生物体也可能是蒸汽加热的可接受替代物。
