Department of Dermatology and Skin Science, University of British Columbia, Vancouver, BC.
J Cutan Med Surg. 2011 Mar-Apr;15(2):103-10. doi: 10.2310/7750.2011.10014.
The extracellular protein collagen triple helix repeat containing 1 (CTHRC1) is aberrantly upregulated in melanoma and most human solid cancers. However, its role in cancer remains unknown.
In this study, we investigated the functional impact of CTHRC1 on melanoma cells in vitro.
Stable clones of cultured melanoma cells expressing different amounts of CTHRC1 protein were generated and evaluated to characterize their growth, survival, and attachment ability as well as their sensitivity to chemotherapy.
In cultured MMAN and MMRU melanoma cells, increased expression of CTHRC1 protein resulted in morphologic cell changes, enhanced cell adhesion to culture surfaces, increased cell proliferation, and decreased apoptosis. Furthermore, decreased CTHRC1 expression through antisense inhibition enhanced temozolomide sensitivity.
CTHRC1 expression influences cellular processes, including cell adhesion and survival. Additionally, CTHRC1 inhibition may represent a potential method for decreasing melanoma resistance to conventional chemotherapy.
细胞外蛋白三螺旋重复胶原蛋白 1（CTHRC1）在黑色素瘤和大多数人类实体瘤中异常上调。然而，其在癌症中的作用尚不清楚。
本研究旨在研究 CTHRC1 对体外黑色素瘤细胞的功能影响。
生成并评估表达不同量 CTHRC1 蛋白的培养黑色素瘤细胞的稳定克隆，以表征其生长、存活和附着能力以及对化疗的敏感性。
在培养的 MMAN 和 MMRU 黑色素瘤细胞中，CTHRC1 蛋白表达增加导致形态细胞变化，增强细胞与培养表面的粘附性，增加细胞增殖，减少细胞凋亡。此外，通过反义抑制降低 CTHRC1 表达可增强替莫唑胺的敏感性。
CTHRC1 的表达影响细胞过程，包括细胞黏附和存活。此外，抑制 CTHRC1 可能代表降低黑色素瘤对常规化疗耐药性的潜在方法。
