Isaksson-Mettävainio Martin, Van Guelpen Bethany, Oberg Ake, Stenling Roger, Palmqvist Richard, Henriksson Maria L
Department of Medical Biosciences and Pathology, Umeå University, SE-901 85 Umeå, Sweden.
Mol Med Rep. 2008 Nov-Dec;1(6):787-90. doi: 10.3892/mmr_00000029.
c-Met is a receptor tyrosine kinase that has been implicated in the pathogenesis and growth of a wide variety of human malignancies, including CRC, but its role in metastasis is largely unknown. We compared c-Met expression in primary human colorectal carcinomas and distant metastases from the same patients. Formalin-fixed paraffin-embedded tissue samples from 69 colorectal cancer patients were obtained. The protein expression of c-Met was evaluated immunohistochemically using a commercial antibody. The difference in expression between primary tumors and their corresponding distant metastases was analyzed using the Wilcoxon signed-rank test. c-Met expression was statistically significantly lower in the distant metastases compared to their corresponding primary tumors (p<0.001), whereas no difference was found between lymph node metastases and their corresponding primary tumors (p=0.957). The degree of c-Met expression was not related to clinicopathological characteristics such as tumor grade and Dukes' stage at the time of primary tumor diagnosis, or to the location of the distant metastases. We demonstrated that c-Met expression is often reduced in distant metastases compared to their corresponding primary colorectal tumors. Additional studies of c-Met activation and signal transduction will increase our knowledge about the role of c-Met in colorectal cancer metastasis.
c-Met是一种受体酪氨酸激酶，已被证实与包括结直肠癌（CRC）在内的多种人类恶性肿瘤的发病机制和生长有关，但其在转移过程中的作用仍 largely unknown。我们比较了原发性人类结直肠癌及其来自同一患者的远处转移灶中c-Met的表达情况。获取了69例结直肠癌患者的福尔马林固定石蜡包埋组织样本。使用商业抗体通过免疫组织化学方法评估c-Met的蛋白表达。采用Wilcoxon符号秩检验分析原发性肿瘤与其相应远处转移灶之间的表达差异。与相应的原发性肿瘤相比，远处转移灶中c-Met的表达在统计学上显著降低（p<0.001），而淋巴结转移灶与其相应的原发性肿瘤之间未发现差异（p=0.957）。c-Met的表达程度与原发性肿瘤诊断时的肿瘤分级和Dukes分期等临床病理特征无关，也与远处转移灶的位置无关。我们证明，与相应的原发性结直肠肿瘤相比，远处转移灶中c-Met的表达通常会降低。对c-Met激活和信号转导的进一步研究将增加我们对c-Met在结直肠癌转移中作用的认识。
