Denis P, Elena P P, Nordmann J P, Saraux H, Lapalus P
Department of Pharmacology, Faculté de Médecine, Nice, France.
Neurosci Lett. 1990 Aug 14;116(1-2):81-6. doi: 10.1016/0304-3940(90)90390-u.
The localization of dopamine binding sites was studied by in vitro autoradiography in the normal human retina using [125I]SCH 23982 for D1 receptor labelling and [125I]iodosulpride for D2 receptors. Results demonstrated that both types of binding sites were present in human retina. Binding of [125I]SCH 23982 to D1 dopamine receptor was blocked by 1 microM SKF 38393, SCH 23390 (D1 specific compounds) whereas bromocriptine and domperidone (D2 specific compounds) were inactive at the same concentration. On the contrary, binding of [125I]iodosulpride to D2 dopamine receptor was inhibited only by D2 drugs. Precise cellular distribution was given by microautoradiographic techniques and showed that binding sites were exclusively localized to the plexiform layers.
利用[125I]SCH 23982标记D1受体以及[125I]碘舒必利标记D2受体，通过体外放射自显影技术在正常人视网膜中研究多巴胺结合位点的定位。结果表明，两种类型的结合位点均存在于人类视网膜中。[125I]SCH 23982与D1多巴胺受体的结合可被1微摩尔的SKF 38393、SCH 23390（D1特异性化合物）阻断，而溴隐亭和多潘立酮（D2特异性化合物）在相同浓度下无活性。相反，[125I]碘舒必利与D2多巴胺受体的结合仅被D2类药物抑制。通过微放射自显影技术给出了精确的细胞分布，结果显示结合位点仅定位于神经纤维层。
