血清特异性 IgE 定量评估在人体包虫病诊断中的应用。

Quantitative assessment of serum-specific IgE in the diagnosis of human cystic echinococcosis.

机构信息

National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria.

出版信息

Parasite Immunol. 2011 Jul;33(7):371-6. doi: 10.1111/j.1365-3024.2011.01292.x.

DOI:10.1111/j.1365-3024.2011.01292.x

Abstract

Anti-Echinococcus serum immunoglobulin (Ig)E was assessed by the ImmunoCAP system and compared with anti-Echinococcus serum IgG assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. The ImmunoCAP system revealed very high specificity (one false positive of 110 healthy individuals), low cross-reactivity (one false positive of 58 patients with other diseases) and decreased sensitivity (73.55%). Receiver operating characteristic analysis displayed a beneficial diagnostic value with high accuracy. Comparison of the ImmunoCAP system with ELISA and Western blot showed significantly higher specificity and significantly lower cross-reactivity compared with the ELISA. Examination of sera from 155 patients with cystic echinococcosis (CE) showed varying levels of anti-Echinococcus IgE (range, 0.01-118.33 kUA/L). However, most samples had moderately elevated IgE levels. Analysis of serum-specific IgE revealed significantly higher sensitivity of the ImmunoCAP system and significantly higher antibody levels in hepatic CE compared with pulmonary CE.

摘要

采用免疫捕获法（ImmunoCAP）系统检测抗细粒棘球蚴血清免疫球蛋白 E（IgE），并与酶联免疫吸附试验（ELISA）和免疫印迹法检测的抗细粒棘球蚴 IgG 进行比较。ImmunoCAP 系统显示出非常高的特异性（110 例健康个体中仅有 1 例假阳性）、低的交叉反应性（58 例其他疾病患者中仅有 1 例假阳性）和较低的敏感性（73.55%）。受试者工作特征分析显示具有较高的准确性，具有良好的诊断价值。ImmunoCAP 系统与 ELISA 和免疫印迹法的比较显示，与 ELISA 相比，特异性显著提高，交叉反应性显著降低。对 155 例囊性包虫病（CE）患者的血清进行检查，结果显示抗细粒棘球蚴 IgE 水平不同（范围：0.01-118.33 kUA/L）。然而，大多数样本的 IgE 水平中等升高。血清特异性 IgE 分析显示，ImmunoCAP 系统的敏感性显著提高，肝 CE 患者的抗体水平显著高于肺 CE 患者。