Department of Oral and Maxillofacial Surgery, Guanghua School and Research Institute of Stomatology, Sun Yat-sen University, Guangzhou, China.
J Oral Pathol Med. 2011 Aug;40(7):545-51. doi: 10.1111/j.1600-0714.2011.01041.x. Epub 2011 Apr 12.
Tumor budding is a readily detectable histopathological feature and has been recognized as an adverse prognostic factor in several human cancers. However, the prognostic value of tumor budding in tongue squamous cell carcinoma (TSCC) has not been reported. The purpose of this study was to assess the correlation of tumor budding with the clinicopathologic features, and the known molecular biomarkers (E-cadherin and Vimentin), as well as to evaluate its prognostic significance for TSCC.
Archival clinical samples of 230 patients with TSCC were examined for tumor budding. Immunohistochemistry analyses were performed to examine the expression of E-cadherin and Vimentin. Statistical analyses were carried out to assess the correlation of tumor budding with clinicopathologic parameters and patient survival. The potential association between tumor budding and alterations of E-cadherin and Vimentin expression was also assessed.
Of the 230 TSCC cases examined, tumor budding was observed in 165 cases (71.7%), with a mean tumor bud count of 7.5 (range from 1 to 48 buds). High-intensity budding (≥5 tumor buds) was observed in 111 cases (48.3%). Statistical analysis revealed that tumor budding was associated with tumor size (P < 0.05), differentiation (P < 0.05), clinical stage (P < 0.05), lymph node metastasis (P < 0.01), and correlated with reduced overall survival. In addition, significant associations were observed among tumor budding and the deregulation of E-cadherin (P < 0.001) and Vimentin (P < 0.001).
Tumor budding, which associates with epithelial-mesenchymal transition, is a frequent event and appears to be an independent prognostic factor in TSCC.
肿瘤芽突是一种易于检测的组织病理学特征，已被认为是几种人类癌症的不良预后因素。然而，肿瘤芽突在舌鳞状细胞癌（TSCC）中的预后价值尚未报道。本研究旨在评估肿瘤芽突与临床病理特征以及已知的分子生物标志物（E-钙黏蛋白和波形蛋白）的相关性，并评估其对 TSCC 的预后意义。
检查了 230 例 TSCC 患者的存档临床样本，以检查肿瘤芽突。进行免疫组织化学分析以检查 E-钙黏蛋白和波形蛋白的表达。进行统计分析以评估肿瘤芽突与临床病理参数和患者生存的相关性。还评估了肿瘤芽突与 E-钙黏蛋白和波形蛋白表达改变之间的潜在关联。
在检查的 230 例 TSCC 病例中，观察到 165 例（71.7%）存在肿瘤芽突，平均肿瘤芽突数为 7.5（范围为 1 至 48 个芽突）。高强度芽突（≥5 个肿瘤芽突）见于 111 例（48.3%）。统计分析显示，肿瘤芽突与肿瘤大小（P<0.05）、分化（P<0.05）、临床分期（P<0.05）、淋巴结转移（P<0.01）有关，并且与总生存时间降低相关。此外，还观察到肿瘤芽突与 E-钙黏蛋白（P<0.001）和波形蛋白（P<0.001）的失调之间存在显著相关性。
肿瘤芽突与上皮间质转化有关，是一种常见事件，似乎是 TSCC 的独立预后因素。
