Unit of Research in Organismal Biology (URBO), University of Namur, Rue de Bruxelles, 61, 5000 Namur, Belgium.
Mol Ecol Resour. 2011 May;11(3):573-7. doi: 10.1111/j.1755-0998.2010.02963.x. Epub 2011 Jan 14.
Reliable markers are needed to identify the lineages in the invasive clam genus Corbicula. Previous studies have demonstrated that mitochondrial (mt) DNA poorly resolves Corbicula phylogeny, owing to its androgenetic reproductive mode. Moreover, hybridization and mitochondrial/nuclear mismatches occur. We developed the first eleven polymorphic markers to detect these phenomena and to investigate the nuclear identity of Corbicula populations. These microsatellite loci revealed three main lineages in Western Europe. One locus allowed rapid discrimination of these three lineages on agarose gel, saving time and money. Moreover, the eleven markers were successfully cross-amplified in the invasive Corbicula lineages found in North America.
需要可靠的标记来识别入侵蛤蜊属 Corbicul 的谱系。先前的研究表明，由于其雄核发育的繁殖模式，线粒体 (mt) DNA 不能很好地解析 Corbicul 的系统发育。此外，还会发生杂交和线粒体/核不匹配。我们开发了前 11 个多态性标记来检测这些现象，并研究 Corbicul 种群的核身份。这些微卫星位点在西欧揭示了三个主要谱系。一个位点可以在琼脂糖凝胶上快速区分这三个谱系，节省时间和金钱。此外，这 11 个标记在北美的入侵 Corbicul 谱系中成功地进行了交叉扩增。
