[低剂量丙泊酚对食管鳞状细胞癌细胞系Eca109生物学行为的影响]
[Effect of low dose propofol on biological behavior of esophageal squamous cell carcinoma cell line Eca109].
作者信息
Zhang Ling-min, Zhang Ming-xin, Wang Ning, Duan Xiao-yi, Zhou Su-na, Wang Jian-sheng, Jing Gui-xia
机构信息
The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Apr;27(4):402-4.
AIM
To detect the effect of low dose propofol on the proliferation, apoptosis, migration, and invasion of esophageal squamous cell carcinoma cell line Eca109.
METHODS
The proliferation, apoptosis, migration, and invasion of esophageal squamous cell carcinoma cell line Eca109 were detected by MTT assay, flow cytometry, transwell assay respectively. The effect of low dose propofol on expression of heme oxygenase-1 (HO-1) was confirmed by Real-time quantitative PCR.
RESULTS
Low dose propofol could inhibit the proliferation, migration, invasion and promate the apoptosis of esophageal squamous cell carcinoma cell line Eca109. And low dose propofol increased the expression of HO-1 mRNA in a dose-dependment manner.
CONCLUSION
Low dose propofol affects the biological behavior of esophageal squamous cell carcinoma cell line Eca109, which has a relationship with increasing the expression of HO-1.
目的
检测低剂量丙泊酚对食管鳞状细胞癌细胞系Eca109增殖、凋亡、迁移及侵袭的影响。
方法
分别采用MTT法、流式细胞术、Transwell法检测食管鳞状细胞癌细胞系Eca109的增殖、凋亡、迁移及侵袭情况。通过实时定量PCR证实低剂量丙泊酚对血红素加氧酶-1（HO-1）表达的影响。
结果
低剂量丙泊酚可抑制食管鳞状细胞癌细胞系Eca109的增殖、迁移、侵袭并促进其凋亡。且低剂量丙泊酚可呈剂量依赖性增加HO-1 mRNA的表达。
结论
低剂量丙泊酚影响食管鳞状细胞癌细胞系Eca109的生物学行为，这与HO-1表达增加有关。
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