Department of Chemistry, Shanghai Normal University, No. 100, Guilin Road, Shanghai 200234, PR China.
J Colloid Interface Sci. 2011 Jun 15;358(2):392-8. doi: 10.1016/j.jcis.2011.03.051. Epub 2011 Mar 23.
By using the positive charged polystyrene (PS) microsphere as template, mono-disperse amino-(-NH(2)) functionalized hollow silica microspheres (HSMS-NH(2)) with ~1310 nm in diameter and uniform shells were successfully prepared with a modified sol-gel process. The amino functionalized silica were coated on the PS microspheres via ammonia catalysis, co-hydrolysis and condensation of TEOS and APTES, and then the PS templates were selectively dissolved in THF solution to form the functional hollow microspheres. The controllable thickness (35-85 nm) and amino density (2.46×10(-5)-6.18×10(-5) mol/g) of the shells could be facilely tuned by changing the amount of TEOS and APTES. In vitro ultrasound images of as-prepared HSMS-NH(2) with different concentrations in the physiological saline solution were further investigated. The obvious signal enhancement indicates that as-prepared HSMS-NH(2) has a great potential application for ultrasound imaging.
采用带正电荷的聚苯乙烯（PS）微球为模板，通过改进的溶胶-凝胶法成功制备了直径约 1310nm、壳层均匀的单分散氨基（-NH2）功能化空心二氧化硅微球（HSMS-NH2）。通过氨催化、TEOS 和 APTES 的共水解缩合，将氨基功能化的硅烷涂覆在 PS 微球上，然后 PS 模板在四氢呋喃溶液中选择性溶解，形成功能化的空心微球。通过改变 TEOS 和 APTES 的用量，可方便地调节壳层的可控厚度（35-85nm）和氨基密度（2.46×10-5-6.18×10-5mol/g）。进一步研究了在生理盐水中具有不同浓度的所制备的 HSMS-NH2 的体外超声图像。明显的信号增强表明，所制备的 HSMS-NH2 具有很大的超声成像应用潜力。
