母体/胎儿死亡率和胎儿生长受限：一氧化氮和毒力因子在大鼠宫内感染中的作用。
Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats.
机构信息
Department of Neonatal Infectious Diseases, Chair of Neonatology, Poznan University of Medical Sciences, Poznan, Poland.
出版信息
Am J Obstet Gynecol. 2011 Jul;205(1):83.e1-7. doi: 10.1016/j.ajog.2011.02.049. Epub 2011 Feb 23.
OBJECTIVE
The mechanism of infection-related deaths of pregnant rats and intrauterine growth restriction are not understood. We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins.
STUDY DESIGN
Sprague-Dawley rats were infected intrauterinally with the clinical strain of E coli AfaE(+)D(+) or 1 of its isogenic mutants in the presence or absence of the NO synthesis inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME). Maternal/fetal mortality rates, fetoplacental weight, and infection rates were evaluated.
RESULTS
Maternal and/or fetal death was associated with the presence of at least 1 virulence factor (AfaE(+)D(+)>AfaE(+)D(-)>AfaE(-)D(+)) and was increased by L-NAME treatment. The fetal and placental weights were lower than controls and were further reduced by L-NAME treatment.
CONCLUSION
These results demonstrate that NO enhanced AfaE- and AfaD-mediated virulence and plays an important role in Dr/Afa(+)E coli gestational tropism.
目的
目前尚不清楚与感染相关的孕鼠死亡和宫内生长受限的机制。本研究评估了一氧化氮（NO）是否对缺乏 AfaE 或 AfaD 侵袭素的大肠杆菌 Dr/Afa 突变体感染有不同的影响。
研究设计
将临床分离株大肠杆菌 AfaE(+)D(+)或其 1 种同源突变体经宫内感染 Sprague-Dawley 大鼠，同时给予或不给予一氧化氮合成抑制剂 N(G)-硝基-L-精氨酸甲酯（L-NAME）。评估母体/胎儿死亡率、胎仔胎盘重量和感染率。
结果
至少存在 1 种毒力因子（AfaE(+)D(+)>AfaE(+)D(-)>AfaE(-)D(+)）与母体和/或胎儿死亡相关，且 L-NAME 治疗可增加死亡率。胎仔和胎盘重量低于对照组，且 L-NAME 治疗进一步降低了胎仔和胎盘重量。
结论
这些结果表明，NO 增强了 AfaE 和 AfaD 介导的毒力，在 Dr/Afa(+)大肠杆菌的妊娠嗜性中发挥重要作用。
Zotero 插件