微小 RNA-142-3p，RAC1 的新调节因子，抑制肝癌细胞的迁移和侵袭。
MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells.
机构信息
Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
出版信息
FEBS Lett. 2011 May 6;585(9):1322-30. doi: 10.1016/j.febslet.2011.03.067. Epub 2011 Apr 7.
RAC1 regulates a diverse array of cellular events including migration and invasion. MicroRNAs (miRNAs) have a key role in the regulation of gene expression. In this study, we demonstrated that microRNA-142-3p (miR-142-3p) acted as a negative regulator of human RAC1. Overexpression of miR-142-3p decreased RAC1 mRNA and protein levels. Moreover, the overexpression of miR-142-3p suppressed, while blocking of miR-142-3p increased colony formation, migration and invasion in hepatocellular carcinoma (HCC) cell lines (QGY-7703 and SMMC-7721). RAC1 overexpression without the 3'untranslated region abolished the effect of miR-142-3p in the QGY-7703 and SMMC-7721 cells. These results demonstrated that miR-142-3p directly and negatively regulates RAC1 in HCC cells, which highlights the importance of miRNAs in tumorigenesis.
RAC1 调节多种细胞事件，包括迁移和侵袭。MicroRNAs (miRNAs) 在基因表达调控中起着关键作用。在这项研究中，我们证明了 microRNA-142-3p (miR-142-3p) 作为人类 RAC1 的负调节剂。miR-142-3p 的过表达降低了 RAC1 mRNA 和蛋白水平。此外，miR-142-3p 的过表达抑制了肝癌 (HCC) 细胞系 (QGY-7703 和 SMMC-7721) 中的集落形成、迁移和侵袭，而阻断 miR-142-3p 则增加了集落形成、迁移和侵袭。没有 3'非翻译区的 RAC1 过表达消除了 miR-142-3p 在 QGY-7703 和 SMMC-7721 细胞中的作用。这些结果表明，miR-142-3p 直接负调控 HCC 细胞中的 RAC1，这突显了 miRNAs 在肿瘤发生中的重要性。
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