Key Laboratory of Arid and Grassland Ecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, China.
Mycorrhiza. 2012 Jan;22(1):31-9. doi: 10.1007/s00572-011-0378-y. Epub 2011 Apr 12.
Plastic film mulching (PFM) is a widely used agricultural practice in the temperate semi-arid Loess Plateau of China. However, how beneficial soil microbes, arbuscular mycorrhizal (AM) fungi in particular, respond to the PFM practice is not known. Here, a field experiment was performed to study the effects of a 3-month short-term PFM practice on AM fungi in plots planted with spring wheat (Triticum aestivum L. cv. Dingxi-2) in the Loess Plateau. AM colonization, spore density, wheat spike weight, and grain phosphorus (P) content were significantly increased in the PFM treatments, and these changes were mainly attributable to changes in soil properties such as available P and soil moisture. Alkaline phosphatase activity was significantly higher in PFM soils, but levels of AM fungal-related glomalin were similar between treatments. A total of nine AM fungal phylotypes were detected in root samples based on AM fungal SSU rDNA analyses, with six and five phylotypes in PFM and no-PFM plots, respectively. Although AM fungal phylotype richness was not statistically different between treatments, the community compositions were different, with four and three specific phylotypes in the PFM and no-PFM plots, respectively. A significant and rapid change in AM fungal, wheat, and soil variables following PFM suggested that the functioning of the AM symbiosis had been changed in the wheat field under PFM. Future studies are needed to investigate whether PFM applied over a longer term has a similar effect on the AM fungal community and their functioning in an agricultural ecosystem.
塑料薄膜覆盖（PFM）是中国温带半干旱黄土高原广泛应用的农业实践。然而，土壤微生物，特别是丛枝菌根（AM）真菌对 PFM 实践的反应如何尚不清楚。在这里，进行了一项田间试验，以研究在黄土高原种植春小麦（Triticum aestivum L. cv. Dingxi-2）的试验田中，3 个月的短期 PFM 实践对 AM 真菌的影响。AM 定殖、孢子密度、小麦穗重和籽粒磷（P）含量在 PFM 处理中显著增加，这些变化主要归因于土壤性质的变化，如有效磷和土壤水分。PFM 土壤中的碱性磷酸酶活性显著较高，但 AM 真菌相关的胶磷矿含量在处理之间相似。基于 AM 真菌 SSU rDNA 分析，从根样本中检测到 9 种 AM 真菌的系统发育型，PFM 和无 PFM 样地分别有 6 种和 5 种系统发育型。尽管处理之间 AM 真菌系统发育型丰富度没有统计学差异，但群落组成不同，PFM 和无 PFM 样地分别有 4 种和 3 种特定的系统发育型。AM 真菌、小麦和土壤变量在 PFM 后发生了显著而快速的变化，这表明在 PFM 下，小麦田中的 AM 共生功能已经发生了变化。未来的研究需要调查在更长时间内应用 PFM 是否对农业生态系统中的 AM 真菌群落及其功能具有类似的影响。
