水痘带状疱疹病毒转录本的合成与降解。
Synthesis and decay of varicella zoster virus transcripts.
机构信息
Department of Neurology, School of Medicine, University of Colorado School of Medicine, 12700 E 19th Avenue, Box B182, Aurora, CO 80045, USA.
出版信息
J Neurovirol. 2011 Jun;17(3):281-7. doi: 10.1007/s13365-011-0029-2. Epub 2011 Apr 12.
Abstract
Varicella zoster virus (VZV) is highly cell-associated. At least 68 VZV open reading frames (ORFs) are transcribed in varying amounts that increase as infection progresses. Using reverse transcriptase PCR, quantification of total and newly synthesized mRNA showed that ongoing VZV DNA replication is required for continued accumulation of VZV ORF 63, 9, and 40 transcripts. Analysis of stability of 4-thiouridine-labeled transcripts of nine VZV ORFs revealed a similar half-life for all VZV ORFs tested. Thus, difference in mRNA synthesis, and not mRNA decay, is the major factor contributing to the difference in the relative abundance of VZV transcripts in infected cells.
摘要
水痘带状疱疹病毒（VZV）高度细胞相关。在感染过程中，至少有 68 个 VZV 开放阅读框（ORF）以不同的量转录，随着感染的进展而增加。使用逆转录酶 PCR，对总 mRNA 和新合成的 mRNA 的定量表明，VZV ORF63、9 和 40 转录物的持续积累需要持续的 VZV DNA 复制。对九种 VZV ORF 的 4-硫代尿嘧啶标记的转录物的稳定性分析表明，所有测试的 VZV ORF 的半衰期相似。因此，mRNA 合成的差异而不是 mRNA 降解是导致感染细胞中 VZV 转录物相对丰度差异的主要因素。
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