Fagbemi B O, Hillyer G V
University of Puerto Rico School of Medicine, San Juan.
Am J Trop Med Hyg. 1990 Dec;43(6):665-8. doi: 10.4269/ajtmh.1990.43.665.
The Falcon assay screening test-enzyme-linked immunosorbent assay (FAST-ELISA) and the enzyme-linked immunoelectrotransfer blot (EITB) technique were used to test human sera with Dracunculus medinensis adult worm antigen in order to assess their potential value in the immunodiagnosis of dracunculiasis. The human sera used were from patients with prepatent and patent D. medinensis infections or from patients infected with other nematodes (Onchocerca volvulus and Loa loa) or trematodes (Schistosoma mansoni and S. haematobium), as well as uninfected Nigerian and Puerto Rican normal controls. In the FAST-ELISA, the sera from prepatent and patent dracunculiasis patients gave the highest absorbance values relative to normal human sera. The highest cross-reactivity was observed with onchocerciasis sera; no cross-reactivity was seen with sera from individuals with loiasis or schistosomiasis mansoni or haematobia. By the EITB, sera from dracunculiasis patients specifically recognized a 16 kDa protein (Dm 16) and antibodies to Dm 16 disappeared 2 months after worm extraction. Recognition of Dm 16 occurred from the late prepatent stage. A 17 kDa protein (Dm 17) was also recognized by dracunculiasis sera, but antibodies to Dm 17 disappeared more slowly and were present 1 year after recovery. The 16 kDa and 17 kDa antigens of D. medinensis may be useful in the immunodiagnosis of dracunculiasis.
采用猎鹰试验筛选检测-酶联免疫吸附测定法（FAST-ELISA）和酶联免疫电转移印迹法（EITB），以麦地那龙线虫成虫抗原检测人血清，从而评估其在麦地那龙线虫病免疫诊断中的潜在价值。所用人血清来自处于麦地那龙线虫感染前期和发病期的患者，或感染其他线虫（盘尾丝虫和罗阿丝虫）或吸虫（曼氏血吸虫和埃及血吸虫）的患者，以及未感染的尼日利亚和波多黎各正常对照者。在FAST-ELISA中，相对于正常人血清，麦地那龙线虫感染前期和发病期患者的血清吸光度值最高。盘尾丝虫病血清的交叉反应性最高；罗阿丝虫病、曼氏血吸虫病或埃及血吸虫病患者的血清未出现交叉反应。通过EITB检测，麦地那龙线虫病患者的血清特异性识别一种16 kDa蛋白（Dm 16），驱虫2个月后，抗Dm 16抗体消失。从感染前期晚期开始出现对Dm 16的识别。麦地那龙线虫病血清还识别一种17 kDa蛋白（Dm 17），但抗Dm 17抗体消失得更慢，康复1年后仍存在。麦地那龙线虫的16 kDa和17 kDa抗原可能有助于麦地那龙线虫病的免疫诊断。
