Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada.
Small. 2011 May 9;7(9):1219-26. doi: 10.1002/smll.201001829. Epub 2011 Apr 12.
Aptamer-polyelectrolyte microcapsules are prepared for potential use as triggered delivery vehicles and microreactors. The hollow microcapsules are prepared from the sulforhodamine B aptamer and the polyelectrolytes poly(allylamine hydrochloride) and poly(sodium 4-styrene-sulfonate), using layer-by-layer (LbL) film deposition templated on a sacrificial CaCO(3) spherical core. Scanning electron microscopy and confocal microscopy confirm the formation of spherical CaCO(3) cores and LbL-aptamer microcapsules. Colocalization studies with fluorescently-tagged aptamer and sulforhodamine B verify the ability of the aptamer to recognize its cognate target in the presence of the K(+) ions that are required for its characteristic G-quadruplex formation. Fluorescence recovery after photobleaching studies confirms a significant difference in the permeability of the aptamer-polyelectrolyte microcapsules for the sulforhodamine B dye target compared to control microcapsules prepared with a random oligonucleotide. These results suggest that aptamer-based 'smart' responsive films and microcapsules could be applied to problems of catalysis and controlled release.
适体-聚电解质微胶囊被制备用于作为触发式药物输送载体和微反应器的潜在应用。中空微胶囊由荧光素 B 适体和聚电解质聚（盐酸烯丙胺）和聚（4-苯乙烯磺酸钠）组成，使用在牺牲的 CaCO3 球形核模板上的层层（LbL）薄膜沉积来制备。扫描电子显微镜和共焦显微镜证实了 CaCO3 核和 LbL-适体微胶囊的形成。用荧光标记的适体和荧光素 B 的共定位研究验证了适体在其特征 G-四链体形成所需的 K+离子存在下识别其同源靶标的能力。光漂白后荧光恢复研究证实，与用随机寡核苷酸制备的对照微胶囊相比，适体-聚电解质微胶囊对磺基罗丹明 B 染料靶标的渗透性有显著差异。这些结果表明，基于适体的“智能”响应性薄膜和微胶囊可应用于催化和控制释放的问题。
