Ye Ping, Hulan Ya-wen, Huang Dan-yun
First Affiliated Clinical Hospital of Zhejiang Chinese Medical University, Hangzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Mar;31(3):359-62.
To study the effect of Chinese drugs for invigorating qi and tonifying Shen (IQTS) on expression of CD4+CD25+ regulatory T cells in spleen and maternal-fetal interface of abortion-prone mice during pregnancy.
CBA female mice were mated with DBA/2 male mice to establish abortion-prone models, which were randomly divided into 4 groups, the negative control group (fed with normal saline), the positive control group (treated with CsA), the Chinese medicine group (treated with IQTS), and the Chinese and Western medicine group (treated with IQTS+CsA). Mice were sacrificed in batches on the 9th and the 14th day of gestation, their splenic and decidual tissues were taken out to analyse CD4+CD25+ regulatory T-cell expression by flow cytometry.
Compared with the negative control group, the expression of splenic CD4+CD25+ regulatory T all significantly increased on the 9th day of gestation (P < 0.01 or P < 0.05). There was no statistical difference in intergroup comparison of the three treatment groups (P > 0.05). Compared with the negative control group, the expression of splenic CD4+CD25+ regulatory T all significantly increased on the 14th day of gestation (P < 0.01 or P < 0.05). Of them, its expression was the highest in the Chinese and Western medicine group, showing significant difference from that in the Chinese medicine group and the positive group (P < 0.01). The difference between the Chinese medicine group and the positive group was insignificant (P > 0.05). On day 9 of gestation, compared with the negative control group, the expressions of CD4+CD25+ regulatory T in maternal-fetal interface increased in the three treated groups, showing no statistical significance (P > 0.05). Its expression was ordered from high to low in sequence as the Chinese and Western medicine group, the positive control group, the Chinese medicine group, and the negative control group. On day 14 its expression was obviously enhanced in the Chinese and Western medicine group, showing statistical difference from that in the negative control group (P < 0.05). But its expression was obviously enhanced in the Chinese medicine group and the positive group, showing insignificant difference from that in the negative group. The same sequence was found in the percentage of CD4+CD25+ T cells in CD4+ T cells.
Chinese drugs for IQTS could up-regulate the expression of CD4+CD25+ regulatory T in spleen of abortion-prone mice in the early and late pregnancy stages. When combined with CsA, it also could up-regulate its expression in maternal-fetal interface in the mid and late pregnancy stages, suggesting that Chinese drugs for IQTS are facilitate to maintain the immune tolerance state in mice during pregnancy.
研究益气补肾中药对妊娠期间易流产小鼠脾脏及母胎界面CD4+CD25+调节性T细胞表达的影响。
将CBA雌性小鼠与DBA/2雄性小鼠交配建立易流产模型，随机分为4组，即阴性对照组（给予生理盐水）、阳性对照组（给予环孢素A）、中药组（给予益气补肾中药）、中西药组（给予益气补肾中药+环孢素A）。于妊娠第9天和第14天分批处死小鼠，取出脾脏和蜕膜组织，采用流式细胞术分析CD4+CD25+调节性T细胞表达。
与阴性对照组比较，妊娠第9天各治疗组脾脏CD4+CD25+调节性T细胞表达均显著升高（P<0.01或P<0.05）。3个治疗组组间比较差异无统计学意义（P>0.05）。与阴性对照组比较，妊娠第14天各治疗组脾脏CD4+CD25+调节性T细胞表达均显著升高（P<0.01或P<0.05）。其中，中西药组表达最高，与中药组和阳性组比较差异有统计学意义（P<0.01）。中药组与阳性组比较差异无统计学意义（P>0.05）。妊娠第9天，与阴性对照组比较，3个治疗组母胎界面CD4+CD25+调节性T细胞表达均升高，但差异无统计学意义（P>0.05）。其表达由高到低依次为中西药组、阳性对照组、中药组、阴性对照组。妊娠第14天，中西药组表达明显增强，与阴性对照组比较差异有统计学意义（P<0.05）。中药组和阳性组表达也明显增强，但与阴性组比较差异无统计学意义。CD4+T细胞中CD4+CD25+T细胞百分比也呈现相同变化趋势。
益气补肾中药可上调妊娠早、晚期易流产小鼠脾脏CD4+CD25+调节性T细胞表达，与环孢素A联合应用时还可上调妊娠中、晚期母胎界面CD4+CD25+调节性T细胞表达，提示益气补肾中药有助于维持小鼠妊娠期间的免疫耐受状态。
