[身痛逐瘀汤对骨癌痛大鼠疼痛行为及脊髓星形胶质细胞的影响]

[Effect of shentong zhuyu decoction on pain behavior and spinal cord astrocytes model of osteocarcinoma pain].

作者信息

Ren Bing-xu, Ma Zheng-liang, Jin Yan-qing

机构信息

Drum Tower Clinical College of Integorative Medicine, Nanjing University of Traditionnal Chinese Medicine, Nanjing.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Mar;31(3):381-5.

PMID:21485084

Abstract

OBJECTIVE

To study the analgesic effect of shentong zhuyu decoction (SZD) and its effect on the expression of the spinal cord glial fibrillary acidic protein (GFAP).

METHODS

One hundred C3H/HeNCrlVr male mice were randomly divided into the normal group (n=8), the sham operation group (n=30), the model group (n=30), the Chinese medicine (CM) group 1 (n=8), the CM group 2 (n=8), the CM group 3 (n=8), and the vehicle group (n=8). 0.1 g crude drug of SZD/0.4 mL, 0.3 g crude drug of SZD/0.4 mL, 0.9 g crude drug of SZD/0.4 mL, and 0.4 mL normal saline were respectively given by gastrogavage to mice in CM 1, 2, 3 groups and the vehicle group, once daily for seven days starting from Day 14. The paw withdrawal thermal latency (PWTL), as the behavior indicator, was assessed in mice using radiant thermal stimulator. The lumbar enlargement of the spinal cord was taken after the behavioral test on Day 21. GFAP mRNA and protein expressions were detected using real-time quantitative RT-PCR and Western blot.

RESULTS

Compared with the normal group (Day 0) (PWTL: 15.91 +/- 1.65 s) and the sham operation group (PWTL: Day 4: 13.33 +/- 1.44 s; Day 7: 11.28 +/- 0.61 s; Day 10: 15.47 +/- 2.46 s; Day 14: 15.69 +/- 1.98 s; Day 21: 15.69 +/- 1.68 s), the PWTL value in the model group (Day 4: 13.24 +/- 1.02 s; Day 7: 11.30 +/- 1.09 s; Day 10: 9.12 +/- 0.54 s; Day 14: 7.79 +/- 0.77 s; Day 21: 6.36 +/- 0.59 s) progressively decreased (P < 0.05) as time went by, while the spinal cord GFAP mRNA and protein expressions gradually increased. Compared with the normal group (Day 0) and the sham operation group (Day 14), the PWTL value in the CM groups and the vehicle group obviously decreased on Day 14 (P < 0.05). The PWTL value was not significantly different among the model group, CM groups, and the vehicle group on Day 14 (P > 0.05). On Day 21 the PWTL value of CM group 2 and 3 increased and the spinal cord GFAP mRNA and protein expression levels decreased when compared with the model group and the vehicle group (P < 0.05). But no significant difference in the PWTL value or GFAP expression levels was shown among the CM 1 group, the vehicle group, and the model group (P > 0.05).

CONCLUSION

SZD had analgesic effect. Inhibition of the proliferation and activation of the spinal cord astrocytes might be one of its mechanisms.

摘要

目的

研究身痛逐瘀汤（SZD）的镇痛作用及其对脊髓胶质纤维酸性蛋白（GFAP）表达的影响。

方法

将100只C3H/HeNCrlVr雄性小鼠随机分为正常组（n = 8）、假手术组（n = 30）、模型组（n = 30）、中药1组（n = 8）、中药2组（n = 8）、中药3组（n = 8）和溶剂组（n = 8）。从第14天开始，中药1组、2组、3组和溶剂组小鼠分别灌胃给予0.1 g生药/0.4 mL、0.3 g生药/0.4 mL、0.9 g生药/0.4 mL的身痛逐瘀汤及0.4 mL生理盐水，每日1次，连续7天。采用辐射热刺激器评估小鼠的 paw withdrawal thermal latency（PWTL）作为行为指标。在第21天进行行为测试后取脊髓腰膨大。采用实时定量RT-PCR和Western blot检测GFAP mRNA和蛋白表达。

结果

与正常组（第0天）（PWTL：平均值±标准差，15.91 ± 1.65秒）和假手术组（PWTL：第4天：13.33 ± 1.44秒；第7天：11.28 ± 0.61秒；第10天：15.47 ± 2.46秒；第14天：15.69 ± 1.98秒；第21天：15.69 ± 1.68秒）相比，模型组（第4天：13.24 ± 1.02秒；第7天：11.30 ± 1.09秒；第10天：9.12 ± 0.54秒；第14天：7.79 ± 0.77秒；第21天：6.36 ± 0.59秒）的PWTL值随时间逐渐降低（P < 0.05），而脊髓GFAP mRNA和蛋白表达逐渐增加。与正常组（第0天）和假手术组（第14天）相比，中药组和溶剂组在第14天的PWTL值明显降低（P < 0.05）。在第14天，模型组、中药组和溶剂组之间的PWTL值无显著差异（P > 0.05）。在第21天，与模型组和溶剂组相比，中药2组和3组的PWTL值升高，脊髓GFAP mRNA和蛋白表达水平降低（P < 0.05）。但中药1组、溶剂组和模型组之间的PWTL值或GFAP表达水平无显著差异（P > 0.05）。