[噬菌体抗体组合文库：用于分析编码肿瘤坏死因子自身抗体可变区的基因片段的应用]
[Combinatorial libraries of phage antibodies: application for analysis of gene segments encoding variable domains of autoantibodies to tumor necrosis factor].
作者信息
Vikhrova M A, Morozova V V, Khlusevich Ia A, Tikunova N V
出版信息
Mol Biol (Mosk). 2011 Jan-Feb;45(1):96-107.
PMID:21485501
Abstract
Twenty unique phage antibodies to human tumor necrosis factor alpha were selected from a naive combinatorial library of human single chain fragment variable. Analysis of gene segments encoding selected antibodies shown that repertoire of variable domains of heavy and light chains included variable domains of both naive autoantibodies and antibodies produced as a result of somatic hypermutagenesis.
摘要
从人单链可变片段的天然组合文库中筛选出20种针对人肿瘤坏死因子α的独特噬菌体抗体。对编码所选抗体的基因片段进行分析表明，重链和轻链可变结构域的库中既包括天然自身抗体的可变结构域，也包括体细胞超突变产生的抗体的可变结构域。
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