Ma Chao, He Da-wei, Zhao Dan, He Wen-fei, Zhang Yong-bo, Wei Guang-hui
Key Laboratory for Children's Development and Disorders of the Chinese Ministry of Education/Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
Zhonghua Nan Ke Xue. 2011 Mar;17(3):208-13.
To observe the effects of flutamide (Flu) on the development of testicular germocytes in SD rats, and to establish a rat model for further researches on the maldevelopment of cryptorchidism gonocytes (Go).
Pregnant SD rats were subcutaneously injected with Flu from gestational day (GD) 12 to 21 to establish a model of cryptorchidism. The testes of the newborns were harvested on postnatal day (PD) 1, 10, 20 and 80 for observation of their morphological and histological changes by HE staining and detection of the expression of neural cell adhesion molecules (NCAM) by immunohistochemistry and RT-PCR.
Flu induced 43.9% (29/66) of cryptorchidism in the exposed rats. Significant differences were found in the testicular weight and organ coefficient between the Flu and the control groups on PD 20 and 80. Gos remained in the center of seminiferous tubules of the Flu-induced testis on PD 10, and in the center of seminiferous tubules in the cryptorchids' testicular tissues on PD 20 and 80. Immunohistochemistry showed the expression of NCAM on the membrane of the remaining Gos, and RT-PCR revealed significantly up-regulated expression of NCAM mRNA in the Flu-induced testes on PD 10 and 20 as compared with the controls (P < 0.05).
A rat model of Flu-induced cryptorchidism with remaining Gos was successfully established, which could be used to study the mechanism and management of the maldevelopment of cryptorchidism gonocytes.
观察氟他胺（Flu）对SD大鼠睾丸生殖细胞发育的影响，建立大鼠隐睾生殖母细胞（Go）发育异常进一步研究的模型。
妊娠SD大鼠于妊娠第12天至21天皮下注射Flu以建立隐睾模型。在出生后第1天、10天、20天和80天采集新生大鼠的睾丸，通过苏木精-伊红（HE）染色观察其形态和组织学变化，采用免疫组织化学和逆转录-聚合酶链反应（RT-PCR）检测神经细胞黏附分子（NCAM）的表达。
Flu诱导暴露大鼠隐睾发生率为43.9%（29/66）。在出生后第20天和80天，Flu组与对照组的睾丸重量和器官系数存在显著差异。在出生后第10天，Go位于Flu诱导睾丸的生精小管中央，在出生后第20天和80天位于隐睾大鼠睾丸组织的生精小管中央。免疫组织化学显示剩余Go细胞膜上有NCAM表达，RT-PCR显示与对照组相比，出生后第10天和20天Flu诱导睾丸中NCAM mRNA表达显著上调（P<0.05）。
成功建立了Flu诱导的伴有剩余Go的隐睾大鼠模型，可用于研究隐睾生殖母细胞发育异常的机制和处理。
