[T3对氟他胺诱导的隐睾大鼠中发育不良的生殖母细胞（Go）及神经细胞黏附分子（NCAM）表达的调控]

[The regulation of T3 on maldeveloped gonocyte (Go) and expression of NCAM in flutamide-induced cryptorchidism rat].

作者信息

Ma Chao, He Da-Wei, Jiang Yong-Chao, Zhao Dan, He Wen-Fei, Wei Guang-Hui

机构信息

Ministry of Education Key Laboratory of Child Development and Disorders, Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Sep;42(5):638-41.

PMID:22007488

Abstract

OBJECTIVE

To investigate the regulation of T3 on the maldeveloped gonocytes (Go) and the expression of NCAM in Flutamide-induced cryptorchidism SD rat.

METHODS

Postnatal day (PD) 1 and PD20 cryptorchidism SD rat induced by Flu were treated with 15 microg/(kg x d) T3 by subcutaneous injections. Incidence of the cryptorchidism, histology were evaluated. And the expression of NCAM was detected by immunohistochemisty and RT-PCR.

RESULTS

The incidence of cryptorchidism in the group of Flu-induced offsprings treated by 15 microg/(kg x d) T3 from PD1 was 25% (8/32), which was lower than that of Flu group 40.9% (9/22). No Go and NCAM expression were observed in the seminiferous tubules. NCAM mRNA expression in Flu-induced PD13, PD20 testes was up-regulated when compared with control (P < 0.01, P < 0.05), while after treated with 15 microg/ (kg x d) T3 the NCAM expression was significantly down-regulated compared to that of non T3 treated group (P < 0.01), and was not significantly different from that of control (P > 0.05).

CONCLUSION

The incidence of Flu-induced cryptorchidism can be decreased by T3 treatment after birth. T3 may contribute to the down-regulation of the NCAM expression, and reduction or elimination of the remainded gonocytes in crytorcidism seminiferous tubules.

摘要

目的

探讨T3对氟他胺诱导的隐睾SD大鼠中发育不良的生殖母细胞（Go）的调控作用以及神经细胞黏附分子（NCAM）的表达。

方法

对出生后第1天（PD1）和第20天（PD20）由氟他胺诱导的隐睾SD大鼠，通过皮下注射给予15μg/（kg·d）的T3进行治疗。评估隐睾的发生率及组织学情况。通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测NCAM的表达。

结果

从PD1开始用15μg/（kg·d）T3治疗的氟他胺诱导后代组中隐睾的发生率为25%（8/32），低于氟他胺组的40.9%（9/22）。在生精小管中未观察到Go和NCAM的表达。与对照组相比，氟他胺诱导的PD13、PD20睾丸中NCAM mRNA表达上调（P<0.01，P<0.05），而用15μg/（kg·d）T3治疗后，与未用T3治疗组相比，NCAM表达显著下调（P<0.01），且与对照组无显著差异（P>0.05）。