碳纳米管在正常人支气管上皮细胞中的毒性评价。
Toxicity evaluation of carbon nanotubes in normal human bronchial epithelial cells.
机构信息
Nanomaterial Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M. G. Marg, Lucknow 226001, India.
出版信息
J Biomed Nanotechnol. 2011 Feb;7(1):108-9. doi: 10.1166/jbn.2011.1225.
Carbon nanotubes (CNTs) are well known for their exceptional thermal, mechanical and electrical properties. For many CNT applications it is of the foremost importance to know their health hazards related to their exposure. Normal human bronchial epithelial cells (BEAS-2B) has been used for assessment the cytotoxicity of SWCNT (Diameter--1.2-1.5 nm) and DWCNT (Diameter--1.3-5 nm). Clear interference of CNTs with conventional in vitro cytotoxicity assays (MTT, NRU and LDH) dye was found which was confirmed by acellular system. However morphological changes and flow cytometry showed the characteristics of cytotoxicity. Thus our study showed that there is a need of appropriate method for the assessment of cytotoxicity of CNT.
碳纳米管（CNTs）以其优异的热学、力学和电学性能而闻名。对于许多 CNT 应用，了解与暴露相关的健康危害至关重要。通常使用人支气管上皮细胞（BEAS-2B）来评估单壁碳纳米管（直径 1.2-1.5nm）和双壁碳纳米管（直径 1.3-5nm）的细胞毒性。研究发现，CNTs 明显干扰了传统的体外细胞毒性测定（MTT、NRU 和 LDH）染料，这一结果在无细胞系统中得到了证实。然而，形态变化和流式细胞术显示出了细胞毒性的特征。因此，我们的研究表明，需要一种合适的方法来评估 CNT 的细胞毒性。
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