Yoshimura A, Fujitsuka N, Sokabe M, Naruse K, Nomura K, Diwan F H, Ito F
Department of Physiology, Nagoya University School of Medicine, Japan.
J Anat. 1990 Oct;172:89-101.
Intrafusal muscle fibres from bull-frog semitendinosus, iliofibularis and sartorius muscles were classified into three types using the histochemical, immunofluorescent and morphological characteristics, with reference to the extrafusal muscle fibres, which were classified into five types in accordance with Rowlerson & Spurway (1988). Immunofluorescent reactions with antibodies against slow or fast myosins obtained from anterior or posterior latissimus dorsi muscles (ALD or PLD), respectively, of chicken were used as the primary criterion. Histochemical profiles of muscle fibres were classified into nine types of myosin ATPase activity as the secondary criterion. Anti-PLD intrafusal fibres (polar zone) with ATPase profiles of moderate to high acid and alkaline stabilities correspond to large nuclear bag fibres in the classification of Diwan & Ito (1989), whereas anti-ALD fibres (polar zone) with alkaline-labile ATPase profiles correspond to medium nuclear bag fibres. On the basis of diameter, anti-PLD fibres (polar zone) with ATPase profiles of moderate to low acid stability and moderate to high alkaline stability seem to correspond to two types of small nuclear chain fibre. Variations between muscles, between intra- and extrafusal fibres and also between zones along intrafusal fibres are discussed.
参照罗尔森和斯普韦（1988年）将蛙半腱肌、髂腓肌和缝匠肌的梭外肌纤维分为五种类型的方法，利用组织化学、免疫荧光和形态学特征，将牛蛙半腱肌、髂腓肌和缝匠肌的肌梭内肌纤维分为三种类型。分别用针对鸡背阔肌前侧或后侧（ALD或PLD）获取的慢肌球蛋白或快肌球蛋白的抗体进行免疫荧光反应，作为主要标准。肌纤维的组织化学特征分为九种肌球蛋白ATP酶活性类型，作为次要标准。具有中度至高酸稳定性和碱稳定性ATP酶特征的抗PLD梭内纤维（极区），在迪万和伊藤（1989年）的分类中对应于大核袋纤维，而具有碱不稳定ATP酶特征的抗ALD纤维（极区）对应于中核袋纤维。基于直径，具有中度至低酸稳定性和中度至高碱稳定性ATP酶特征的抗PLD纤维（极区）似乎对应于两种小核链纤维。本文讨论了不同肌肉之间、梭内和梭外纤维之间以及沿梭内纤维不同区域之间的差异。
