Lv Feng, Gao Yu-Feng, Zhang Zhen-Huan, Zhang Tian-Chen, Pan Fa-Ming, Cui Ming-Fang, Xia Shu-Ling, Li Xu, Yin Hua-Fa
Feng Lv, Yu-Feng Gao, Zhen-Huan Zhang, Ming-Fang Cui, Shu-Ling Xia, Xu Li, Hua-Fa Yin, Department of Infectious Diseases, the First Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
World J Hepatol. 2011 Mar 27;3(3):72-8. doi: 10.4254/wjh.v3.i3.72.
To investigate the association between the programmed death-1(PD-1) polymorphisms and genetic susceptibility of chronic hepatitis B virus (HBV) infection in Chinese patients.
Two single nucleotide polymorphisms (SNPs), PD-1.1 G > A and PD-1.2 G > A, were genotyped in 539 patients with chronic HBV infection and 353 other family members (HbsAg-) from 256 nuclear families using polymerase chain reactiorestriction fragment length polymorphisms assay. The associations between PD-1 polymorphisms and genetic susceptibility of chronic HBV infection were analyzed usng the family-based association analysis method.
No association or linkage was detected among 539 patients. Univariate (single-marker) family-based association tests demonstrated that PD-1 genotypes, alleles and transmitted haplotypes are not associated with chronic HBV infection (all with P value more than 0.05). Transmission/disequilibrium test and sibship disequilibrium test analysis showed no excess of the alleles from heterozygous parents to affected offspring (P = 0.688880, P = 1.000000 respectively).
The data demonstrated that PD-1.1 and PD-1.2 polymorphisms are not associated with chronic HBV infection in Chinese patients.
研究程序性死亡因子1（PD-1）基因多态性与中国慢性乙型肝炎病毒（HBV）感染患者遗传易感性之间的关联。
采用聚合酶链反应-限制性片段长度多态性分析方法，对来自256个核心家庭的539例慢性HBV感染患者及353名其他家庭成员（乙肝表面抗原阴性）的两个单核苷酸多态性（SNP），即PD-1.1 G>A和PD-1.2 G>A进行基因分型。采用基于家系的关联分析方法分析PD-1基因多态性与慢性HBV感染遗传易感性之间的关联。
在539例患者中未检测到关联或连锁。单变量（单标记）基于家系的关联检验表明，PD-1基因型、等位基因和传递单倍型与慢性HBV感染无关（P值均大于0.05）。传递/不平衡检验和同胞不平衡检验分析显示，杂合子父母向受影响后代传递的等位基因没有过剩（P值分别为0.688880和1.000000）。
数据表明，PD-1.1和PD-1.2基因多态性与中国慢性HBV感染患者无关。
